EFFECTS OF 4-HYDROXY TEMPO (TEMPOL) ADDED TO TRIS-CITRIC ACID BASED EXTENDER ON THE PRESERVABILITY OF BUFFALO BULL SPERMATOZOA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TABASSOMI M.; ALAVI SHOUSHTARI S.M.; DOROSTKAR K.; KHAKI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE) Year:2014 | Volume:7 | Issue:2 (21) Page(s): 9-19

Download Full-Text

Persian Verion

View:

700

Download:

Cites:

Information Journal Paper

Title

EFFECTS OF 4-HYDROXY TEMPO (TEMPOL) ADDED TO TRIS-CITRIC ACID BASED EXTENDER ON THE PRESERVABILITY OF BUFFALO BULL SPERMATOZOA

Author(s)

TABASSOMI M. | ALAVI SHOUSHTARI S.M. | DOROSTKAR K. | KHAKI A. | Issue Writer Certificate

Keywords

BUFFALOQ3

SEMENQ2

ANTIOXIDANTQ1

4-HYDROXY TEMPOQ2

Abstract

The aim of the present study is to investigate the effects of in vitro supplementation of Tempol on spermatozoa of BUFFALO bills before and after freeze-thawing. A total of 25 samples from five healthy BUFFALO bulls (5 ejaculates from each bull on 5 different occasions) were used. Each ejaculate was diluted at 37°C in tris-citric acid based extender (without egg yolk) containing 0 (control), 0.5, 1, 4, 8 and 12 mM Tempol. Sperm progressive motility and viability were evaluated in diluted SEMEN at 0 (T0) (immediately after dilution), 60 (T1) and 120 (T2) minutes after SEMEN dilution. In the next step, the same doses of Tempol, egg yolk and glycerol were added to the above extender and SEMEN samples diluted, cooled to 4˚C within 2 h, equilibrated for 4 hours and sperm parameters (progressive motility, viability, membrane integrity and DNA damage) estimated. The equilibrated SEMEN was packed into 0.5 mL French straws and frozen in liquid nitrogen. Later, the SEMEN was thawed and analyzed for the same parameters, as well as total ANTIOXIDANT capacity (TAC). Results showed that adding 0.5 and 1 mM Tempol to the SEMEN extender preserved the sperm progressive motility in diluted and equilibrated SEMEN compared to the control without affecting other parameters. However, in frozen-thawed SEMEN, extenders containing 0.5 and 1 mM Tempol significantly preserved sperm progressive motility, viability, membrane integrity and SEMEN total ANTIOXIDANT capacity and also resulted in lower DNA damaged sperm counts. It was concluded that addition of 0.5 or 1mM tempol to the extender would preserve SEMEN quality during freeze-thawing.

Cites

No record.

References

No record.

Cite

APA: Copy

TABASSOMI, M., ALAVI SHOUSHTARI, S.M., DOROSTKAR, K., & KHAKI, A.. (2014). EFFECTS OF 4-HYDROXY TEMPO (TEMPOL) ADDED TO TRIS-CITRIC ACID BASED EXTENDER ON THE PRESERVABILITY OF BUFFALO BULL SPERMATOZOA. JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE), 7(2 (21)), 9-19. SID. https://sid.ir/paper/175491/en

Vancouver: Copy

TABASSOMI M., ALAVI SHOUSHTARI S.M., DOROSTKAR K., KHAKI A.. EFFECTS OF 4-HYDROXY TEMPO (TEMPOL) ADDED TO TRIS-CITRIC ACID BASED EXTENDER ON THE PRESERVABILITY OF BUFFALO BULL SPERMATOZOA. JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE)[Internet]. 2014;7(2 (21)):9-19. Available from: https://sid.ir/paper/175491/en

IEEE: Copy

M. TABASSOMI, S.M. ALAVI SHOUSHTARI, K. DOROSTKAR, and A. KHAKI, “EFFECTS OF 4-HYDROXY TEMPO (TEMPOL) ADDED TO TRIS-CITRIC ACID BASED EXTENDER ON THE PRESERVABILITY OF BUFFALO BULL SPERMATOZOA,” JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE), vol. 7, no. 2 (21), pp. 9–19, 2014, [Online]. Available: https://sid.ir/paper/175491/en

Related Journal Papers