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Information Journal Paper

Title

EFFECTS OF ADDITION OF GLUTAMINE TO SEMEN EXTENDERS ON MICROSCOPIC FACTORS OF BUFFALO BULLS SPERM AFTER THAWING

Pages

  727-731

Abstract

 The aim of this study was to evaluate the effects of the addition of different levels of antioxidant (glutamine) on BUFFALO SPERMatozoa cryopreserved in an egg yolk based extender. Ejaculates were collected From 5 matures BUFFALO bulls (four ejaculate per BUFFALO), were evaluated and diluted at 37oC in one of the following experiments: Tris-egg yolk extender (control), or the same extender supplemented with 10, 25, 50, 75 or 100 mM GLUTAMINE. The semen was loaded into 0.5 ml straws, cooled and frozen in a manual freezer and subsequently stored in liquid nitrogen. Prior to evaluation, frozen straws were thawed in a water bath (37oC for 20 s). Freezing extenders supplemented with 25 mM GLUTAMINE led to higher SPERM motility values, compared to the control and other groups. The addition of antioxidants did not affect acrosomal integrity compared to the control. The percentage of SPERM viability in extender supplemented with 25 mM GLUTAMINE was higher than control group (78.03±0.37 & 53.13±0.40, respectively). The SPERM viability in extender supplemented with 100 mM GLUTAMINE was lower than other GLUTAMINE supplemented groups. In conclusion, addition of GLUTAMINE to BUFFALO semen extender laded to higher frozen SPERM quality in BUFFALO.

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    APA: Copy

    BEHESHTI, R., YOSEFI ASL, M., & GHIASI GHALEKANDI, J.. (2012). EFFECTS OF ADDITION OF GLUTAMINE TO SEMEN EXTENDERS ON MICROSCOPIC FACTORS OF BUFFALO BULLS SPERM AFTER THAWING. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 9 (NEW)(3 (38)), 727-731. SID. https://sid.ir/paper/197363/en

    Vancouver: Copy

    BEHESHTI R., YOSEFI ASL M., GHIASI GHALEKANDI J.. EFFECTS OF ADDITION OF GLUTAMINE TO SEMEN EXTENDERS ON MICROSCOPIC FACTORS OF BUFFALO BULLS SPERM AFTER THAWING. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2012;9 (NEW)(3 (38)):727-731. Available from: https://sid.ir/paper/197363/en

    IEEE: Copy

    R. BEHESHTI, M. YOSEFI ASL, and J. GHIASI GHALEKANDI, “EFFECTS OF ADDITION OF GLUTAMINE TO SEMEN EXTENDERS ON MICROSCOPIC FACTORS OF BUFFALO BULLS SPERM AFTER THAWING,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 9 (NEW), no. 3 (38), pp. 727–731, 2012, [Online]. Available: https://sid.ir/paper/197363/en

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