MAKING A CONSTRUCT CONTAINING OF γ-GLOBIN GENE AND CONTROL REGIONS OF BETA GENE CLUSTER AIMING FOR STUDYING OF EFFECTIVE MUTATIONS IN HPFH PHENOTYPE

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BIKHOUF TORBATI M.; JAMSHIDI FATEMEH; KHANAHMAD H.; ZEYNALI SIROUS; KARIMIPOUR MORTEZA

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Journal: JOURNAL OF ANIMAL BIOLOGY Year:2009 | Volume:1 | Issue:4 Page(s): 1-6

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Title

MAKING A CONSTRUCT CONTAINING OF γ-GLOBIN GENE AND CONTROL REGIONS OF BETA GENE CLUSTER AIMING FOR STUDYING OF EFFECTIVE MUTATIONS IN HPFH PHENOTYPE

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Keywords

β-THALASSEMIAQ3

GAMMA GLOBINQ3

FETAL HEMOGLOBINQ3

HPFHQ3

LCR.Q3

Abstract

In β-thalassemia patients are mutations or deletions in beta globin gene which due to absence or reduction of β-globin chain synthesis. Adult hemoglobin (HbA) contains 2 Alfa Globin and 2 Beta Globin chains (α2β2) while FETAL HEMOGLOBIN (HbF) contains 2 Alfa Globin and 2 GAMMA GLOBIN chains (α2γ2). HbF switches to HbA in early weeks after the birth. A kind of β-thalassemia is HPFH phynotype. In these patients the level of HbF will be high in after the birth continuously. The GAMMA GLOBIN gene is in beta globin gene cluster and its expression will be control by locus control region (LCR) which located in upstream of beta gene cluster. The LCR.composed of five DNase I hyper sensitive sites (HS1-5) in Erythroid cells. We amplified HS2, HS3, HS4 segments (mini LCR. and γ-globin gene from the genomic DNA of a normal individual by PCR. Each of segments cloned in pTZ57R/T vector and then subcloned in the pBGGT vector, a derivative of PUC19 plasmid. This construct is a positive control and containing 5' HS4,HS3,HS2 3' segments (mini LCR. 4 kb) and A Gamma gene (2 kb). That could be used in studying of some mutations which due to HPFH phenotype and affect expression of γ-globin gene and also for induction of HbF production in gene therapy of β-thalassemia.

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APA: Copy

BIKHOUF TORBATI, M., JAMSHIDI, FATEMEH, KHANAHMAD, H., ZEYNALI, SIROUS, & KARIMIPOUR, MORTEZA. (2009). MAKING A CONSTRUCT CONTAINING OF γ-GLOBIN GENE AND CONTROL REGIONS OF BETA GENE CLUSTER AIMING FOR STUDYING OF EFFECTIVE MUTATIONS IN HPFH PHENOTYPE. JOURNAL OF ANIMAL BIOLOGY, 1(4), 1-6. SID. https://sid.ir/paper/176692/en

Vancouver: Copy

BIKHOUF TORBATI M., JAMSHIDI FATEMEH, KHANAHMAD H., ZEYNALI SIROUS, KARIMIPOUR MORTEZA. MAKING A CONSTRUCT CONTAINING OF γ-GLOBIN GENE AND CONTROL REGIONS OF BETA GENE CLUSTER AIMING FOR STUDYING OF EFFECTIVE MUTATIONS IN HPFH PHENOTYPE. JOURNAL OF ANIMAL BIOLOGY[Internet]. 2009;1(4):1-6. Available from: https://sid.ir/paper/176692/en

IEEE: Copy

M. BIKHOUF TORBATI, FATEMEH JAMSHIDI, H. KHANAHMAD, SIROUS ZEYNALI, and MORTEZA KARIMIPOUR, “MAKING A CONSTRUCT CONTAINING OF γ-GLOBIN GENE AND CONTROL REGIONS OF BETA GENE CLUSTER AIMING FOR STUDYING OF EFFECTIVE MUTATIONS IN HPFH PHENOTYPE,” JOURNAL OF ANIMAL BIOLOGY, vol. 1, no. 4, pp. 1–6, 2009, [Online]. Available: https://sid.ir/paper/176692/en

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