EFFECTS OF IN VITRO POLYPLOIDY INDUCTION ON REGENERATED PLANTLETS OF LATTICE HENBANE (HYOSCYAMUS RETICULATUS L.)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MADANI SEYYED HADI; HOSSEINI BAHMAN; Dehghan Esmail; REZAEI CHIYANEH ESMAIL

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CROP BREEDING Year:2016 | Volume:8 | Issue:17 Page(s): 66-76

Download Full-Text

Persian Verion

View:

666

Download:

Cites:

Information Journal Paper

Title

EFFECTS OF IN VITRO POLYPLOIDY INDUCTION ON REGENERATED PLANTLETS OF LATTICE HENBANE (HYOSCYAMUS RETICULATUS L.)

Author(s)

MADANI SEYYED HADI | HOSSEINI BAHMAN | Dehghan Esmail | REZAEI CHIYANEH ESMAIL | Issue Writer Certificate

Keywords

COLCHICINEQ1

FLOW CYTOMETRYQ1

HYOSCYAMUS RETICULATUS L.Q2

IN VITROQ2

TETRAPLOIDYQ2

Abstract

Henbane lattice (Hyoscyamus reticulatus L.), is one of the most important medicinal herbs of the solanaceae family which due to having Tropane alkaloids, frequently used in medicinal industry. Medicinal plant Regeneration, proliferation and IN VITRO ploidy induction are one of the interesting issues in biotechnology and plant tissue culture. This study was arranged as a completely randomized factorial design with Two Factors, BAP in three levels (0, 4.4, 8.8 mM) and IAA in three levels (0, 1.1, 2.2m) with three replications. Then, IN VITRO polyploidy induction in regenerated plants was surveyed by 5 different concentrations of COLCHICINE at (0.00, 0.05, 0.1and 0.2 %) at various exposure times (24, 48 and 72 h). in order to determining plant ploidy level, microscopic, FLOW CYTOMETRY and chromosome counting were performed. The results showed that after six months; the best results (13.13 plantlet in each explants) were obtained in 8.8 mM BAP combined with 2.2 mM IAA. FLOW CYTOMETRY study results showed that highest polyploid plants (72%) were obtained in 0.1% COLCHICINE with 48 h exposure times.Polyploidy induction was associated with significant changes in plant characteristics. The chromosome number of diploid plants was 2n=2x=34 and in tetraploid plants was 2n=4x =68. Therefore, can say that COLCHICINE effectively induce polyploidy in the plant.

Cites

No record.

References

No record.

Cite

APA: Copy

MADANI, SEYYED HADI, HOSSEINI, BAHMAN, Dehghan, Esmail, & REZAEI CHIYANEH, ESMAIL. (2016). EFFECTS OF IN VITRO POLYPLOIDY INDUCTION ON REGENERATED PLANTLETS OF LATTICE HENBANE (HYOSCYAMUS RETICULATUS L.). JOURNAL OF CROP BREEDING, 8(17), 66-76. SID. https://sid.ir/paper/181239/en

Vancouver: Copy

MADANI SEYYED HADI, HOSSEINI BAHMAN, Dehghan Esmail, REZAEI CHIYANEH ESMAIL. EFFECTS OF IN VITRO POLYPLOIDY INDUCTION ON REGENERATED PLANTLETS OF LATTICE HENBANE (HYOSCYAMUS RETICULATUS L.). JOURNAL OF CROP BREEDING[Internet]. 2016;8(17):66-76. Available from: https://sid.ir/paper/181239/en

IEEE: Copy

SEYYED HADI MADANI, BAHMAN HOSSEINI, Esmail Dehghan, and ESMAIL REZAEI CHIYANEH, “EFFECTS OF IN VITRO POLYPLOIDY INDUCTION ON REGENERATED PLANTLETS OF LATTICE HENBANE (HYOSCYAMUS RETICULATUS L.),” JOURNAL OF CROP BREEDING, vol. 8, no. 17, pp. 66–76, 2016, [Online]. Available: https://sid.ir/paper/181239/en

Related Journal Papers