PURIFICATION AND SOME PROPERTIES OF XYLANASE (EC:3.2.1.8) FROM BACILLUS STEAROTHERMOPHILUS (ATCC 12980)

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FAEZIZADEH Z.; GHARIB A.; MIRZAEI MOHSEN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2009 | Volume:1 | Issue:2 (2) Page(s): 51-59

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Title

PURIFICATION AND SOME PROPERTIES OF XYLANASE (EC:3.2.1.8) FROM BACILLUS STEAROTHERMOPHILUS (ATCC 12980)

Author(s)

FAEZIZADEH Z. | GHARIB A. | MIRZAEI MOHSEN | Issue Writer Certificate

Keywords

BACILLUS STEAROTHERMOPHILUSQ3

XYLANASEQ3

PURIFICATIONQ2

CHROMATOGRAPHYQ2

Abstract

Background and Objective: Xylan the major portion of the hemicellulose of plant cell walls are heterogeneous polysaccharides. XYLANASEs (EC:3.2.1.8) are enzymes obtained from different species of microorganisms that degrade the xylosidic linkages of xylan’s backbone producing xylose with other monoresidues. In recent years, XYLANASEs have many application in industry such as paper biobleaching and oil clearing.Materials and methods: In this study XYLANASE enzyme was purified from culture supernatant of BACILLUS STEAROTHERMOPHILUS (ATCC 12980) by ammonium sulfate precipitation, gel filtration on sephadex G- 100 followed by ion exchange CHROMATOGRAPHY on DEAE-cellulose. Also, the effect of pH and temperature on purified XYLANASE activity was studied.Results: In DEAE- cellulose column CHROMATOGRAPHY, three protein peaks F-1a, F-1b and F-1c were appeared. Among these peaks, only F-1b showed XYLANASE activity and the degree of PURIFICATION attained 63.09 fold. The specific activity and PURIFICATION fold of the purified XYLANASE was 87.7U/mg of protein and 17.45, respectively. The enzyme gave maximum activity against xylan as substrate at pH 7.0 and temperature at 60°C. In paper CHROMATOGRAPHY xylose was detected as the hydrolysis products of oat- spelt xylan by the XYLANASE at 16 h. These results indicate that XYLANASE of BACILLUS STEAROTHERMOPHILUS (ATCC 12980) was endoxylanase.Conclusion: These data includes, optimal pH and temprature suggested that purified XYLANASE can be suitable for industrial applications.

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APA: Copy

FAEZIZADEH, Z., GHARIB, A., & MIRZAEI, MOHSEN. (2009). PURIFICATION AND SOME PROPERTIES OF XYLANASE (EC:3.2.1.8) FROM BACILLUS STEAROTHERMOPHILUS (ATCC 12980). JOURNAL OF MICROBIAL BIOTECHNOLOGY, 1(2 (2)), 51-59. SID. https://sid.ir/paper/181408/en

Vancouver: Copy

FAEZIZADEH Z., GHARIB A., MIRZAEI MOHSEN. PURIFICATION AND SOME PROPERTIES OF XYLANASE (EC:3.2.1.8) FROM BACILLUS STEAROTHERMOPHILUS (ATCC 12980). JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2009;1(2 (2)):51-59. Available from: https://sid.ir/paper/181408/en

IEEE: Copy

Z. FAEZIZADEH, A. GHARIB, and MOHSEN MIRZAEI, “PURIFICATION AND SOME PROPERTIES OF XYLANASE (EC:3.2.1.8) FROM BACILLUS STEAROTHERMOPHILUS (ATCC 12980),” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 1, no. 2 (2), pp. 51–59, 2009, [Online]. Available: https://sid.ir/paper/181408/en

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