THE EFFECT OF DIFFERENT DOSES OF GAMMA IRRADIATION ON THE STERILIZATION OF FETAL BOVINE SERUM

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ERFANMANESH A.; GHOLAMPOUR H.; GHARIBI SORAYA

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2011 | Volume:3 | Issue:9 Page(s): 1-6

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Title

THE EFFECT OF DIFFERENT DOSES OF GAMMA IRRADIATION ON THE STERILIZATION OF FETAL BOVINE SERUM

Author(s)

ERFANMANESH A. | GHOLAMPOUR H. | GHARIBI SORAYA | Issue Writer Certificate

Keywords

GAMMA IRRADIATIONQ2

FBSQ2

STERILIZATIONQ2

Abstract

Background and objectives: The in vitro techniques of cell culture are important in biological sciences, medicine, and biotechnology. The presence of many biological substances in cell culture is necessary. Fetal bovine serum (FBS) is the most popular cell culture additive for the stimulation of cellular proliferation and biological production. Since there is a possibility of contamination of animal sera with viruses, bacteria, fungi and immunoglobulin's, the application of methods for the inactivation or removal of these pathogens is extremely important in plasma production.Materials and Methods: The GAMMA IRRADIATION has been used in STERILIZATION of FBS and other biological materials. In the current study, the effect of variable doses of GAMMA IRRADIATION on the STERILIZATION of FBS was investigated and compared to filtration method. Raw fetal bovine serum was prepared from Academic Center for Education, Culture, and Research (ACECR). FBS samples were divided into eight groups including Raw sera, filtrated sera, and 5, 10, 15, 20, 25 and 35 KGray gamma receivers (in 5.6 Gray/s dose rate). Sampling was repeated three times. The specimens were assessed for the sterility.Results: The results showed that there were no bacterial, mycological, mycoplasmal or viral microorganisms in 10 KGray and higher doses of gamma receivers. However, the filtration method failed to complete elimination of the virus.Conclusion: According to the results, it can be concluded that GAMMA IRRADIATION is a confident and useful method in FBS STERILIZATION, and the applicable dose varies based on the product preparation terms and application.

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APA: Copy

ERFANMANESH, A., GHOLAMPOUR, H., & GHARIBI, SORAYA. (2011). THE EFFECT OF DIFFERENT DOSES OF GAMMA IRRADIATION ON THE STERILIZATION OF FETAL BOVINE SERUM. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 3(9), 1-6. SID. https://sid.ir/paper/181420/en

Vancouver: Copy

ERFANMANESH A., GHOLAMPOUR H., GHARIBI SORAYA. THE EFFECT OF DIFFERENT DOSES OF GAMMA IRRADIATION ON THE STERILIZATION OF FETAL BOVINE SERUM. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;3(9):1-6. Available from: https://sid.ir/paper/181420/en

IEEE: Copy

A. ERFANMANESH, H. GHOLAMPOUR, and SORAYA GHARIBI, “THE EFFECT OF DIFFERENT DOSES OF GAMMA IRRADIATION ON THE STERILIZATION OF FETAL BOVINE SERUM,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 3, no. 9, pp. 1–6, 2011, [Online]. Available: https://sid.ir/paper/181420/en

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