PRODUCTION OF PHB BY SOMEMESOPHILE AND THERMOPHILE BACILLUS SPP ISOLATED FROM SOIL

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AKHAVAN SEPAHI ABBAS; HEDAYATI Z.; KHANAFARI A.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2011 | Volume:3 | Issue:9 Page(s): 7-12

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Title

PRODUCTION OF PHB BY SOMEMESOPHILE AND THERMOPHILE BACILLUS SPP ISOLATED FROM SOIL

Author(s)

AKHAVAN SEPAHI ABBAS | HEDAYATI Z. | KHANAFARI A. | Issue Writer Certificate

Keywords

BACILLUSQ2

BIOPOLYMERQ2

MESOPHILEQ2

THERMOPHILEQ1

GC-MASSQ2

Abstract

Background and Objective: Synthetic polymers obtained from petrol causes soil pollution, for this reason, PHB has gained importance since it can easily be dismantled in nature. The microbial poly hydroxyalkanoate family of polyesters is synthesized by a wide variety of bacteria as intracellular carbon and energy storage materials. PHB has been identified in more than 20 bacterial genera, including Azotobacter, BACILLUS, Alcaligenes, Pseudomonas and Rhodospirillum.Materials and Methods: In this study, 11 strains of the genus MESOPHILE BACILLUS and 7 strains of the genus THERMOPHILE BACILLUS isolated from Golestan forest soil. The samples were heated at 75oc for 20 min in water bath, than were diluted in sterile water and were placed on nutrient agar media. Plates were incubated at 30oc and 50oc for 24-48h. PHB production by these strains was determined by the spectrophotometric method and confirmed by GC-MASS and FT-IR.Results: PHB production was found in MESOPHILE strains ranged from 6.72-48.70 % (W/V) and in THERMOPHILE strains 3.84-39.21%. PHB was found in all strain, among them B11 and B4 were capable to produce maximum BIOPOLYMER 48.70% and 39.21%. The best shaker rotation and temperature for B11 are 200 rpm and 32oc and for B4 are 200 rpm and 45oc.Conclusion: B11 and B4 strains are capable to produce BIOPOLYMER and suggested that in the future these strains can be used as one of the producer of BIOPOLYMER. Using of isolated BACILLUS sp in biosynthesing PHB which can make them suitable for applications in the packaging industry and as substitute for hydrocarbon-based plastics.

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APA: Copy

AKHAVAN SEPAHI, ABBAS, HEDAYATI, Z., & KHANAFARI, A.. (2011). PRODUCTION OF PHB BY SOMEMESOPHILE AND THERMOPHILE BACILLUS SPP ISOLATED FROM SOIL. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 3(9), 7-12. SID. https://sid.ir/paper/181426/en

Vancouver: Copy

AKHAVAN SEPAHI ABBAS, HEDAYATI Z., KHANAFARI A.. PRODUCTION OF PHB BY SOMEMESOPHILE AND THERMOPHILE BACILLUS SPP ISOLATED FROM SOIL. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;3(9):7-12. Available from: https://sid.ir/paper/181426/en

IEEE: Copy

ABBAS AKHAVAN SEPAHI, Z. HEDAYATI, and A. KHANAFARI, “PRODUCTION OF PHB BY SOMEMESOPHILE AND THERMOPHILE BACILLUS SPP ISOLATED FROM SOIL,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 3, no. 9, pp. 7–12, 2011, [Online]. Available: https://sid.ir/paper/181426/en

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