COMPARISON OF MOLECULAR TYPING ALLERGENIC FUNGI (ASPERGILLUS FUMIGATUS & FUSARIUM OXYSPORUM) USING COLONY PCR & PCR

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NEVISI F.; BAYAT M.; HASHEMI M.; ROUDBAR MOHAMMADI S.; SAADATMAND S.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2010 | Volume:2 | Issue:6 Page(s): 39-45

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Title

COMPARISON OF MOLECULAR TYPING ALLERGENIC FUNGI (ASPERGILLUS FUMIGATUS & FUSARIUM OXYSPORUM) USING COLONY PCR & PCR

Author(s)

Keywords

ASPERGILLUSQ2

FUSARIUMQ2

COLONY PCRQ2

ALLERGYQ3

ASPF1Q2

ITS4Q2

1Q2

Abstract

Background and Objective: Prevalence of allergic diseases has been increased in recent years. ALLERGY is defined as the response of immune system against external proteins. It has been indicated that spore and conidia of the fungi existing in air play a role in creation of different forms of ALLERGY, especially the respiratory ALLERGY. The typical method for identification of fungi is culture, which requires time for morphological characteristics identification of species. Molecular methods have been developed with the purpose of improving the sensitivity and specificity detection of pathogen and allergenic fungi. The aim of this research was studying on allergenic level of ASPERGILLUS and FUSARIUM by an appropriate and reliable molecular method of COLONY PCR in order to decrease the identification time.Materials and Methods: After culture of ASPERGILLUS and FUSARIUM on PDA medium and decomposition of fungus spores, DNA extraction was carried out using Gen Pajuhan Puya kit, which was subsequently used in PCR as pattern for amplification of the genes Aspf and Its4, 1. The same process was fulfilled in next stage in COLONY PCR without DNA extraction.Results: PCR results indicated that each primer caused a different amplified pattern and two fungi was discriminate by specific primer. In addition both PCR and COLONY PCR was identical. In this study COLONY PCR was applied to filament fungi and led to identical results compared to PCR. As the extraction step is time-consuming, COLONY PCR method is suggested.Conclusion: Through optimization of PCR and COLONY PCR sample infected by ASPERGILLUS, a reliable molecular method can be provided for rapid identification of the mentioned fungi in clinical laboratories.

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APA: Copy

NEVISI, F., BAYAT, M., HASHEMI, M., ROUDBAR MOHAMMADI, S., & SAADATMAND, S.. (2010). COMPARISON OF MOLECULAR TYPING ALLERGENIC FUNGI (ASPERGILLUS FUMIGATUS & FUSARIUM OXYSPORUM) USING COLONY PCR & PCR. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 2(6), 39-45. SID. https://sid.ir/paper/181448/en

Vancouver: Copy

NEVISI F., BAYAT M., HASHEMI M., ROUDBAR MOHAMMADI S., SAADATMAND S.. COMPARISON OF MOLECULAR TYPING ALLERGENIC FUNGI (ASPERGILLUS FUMIGATUS & FUSARIUM OXYSPORUM) USING COLONY PCR & PCR. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2010;2(6):39-45. Available from: https://sid.ir/paper/181448/en

IEEE: Copy

F. NEVISI, M. BAYAT, M. HASHEMI, S. ROUDBAR MOHAMMADI, and S. SAADATMAND, “COMPARISON OF MOLECULAR TYPING ALLERGENIC FUNGI (ASPERGILLUS FUMIGATUS & FUSARIUM OXYSPORUM) USING COLONY PCR & PCR,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 2, no. 6, pp. 39–45, 2010, [Online]. Available: https://sid.ir/paper/181448/en

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