MOLECULAR TYPING OF E. COLI ISOLATES FROM BILE DUCT SAMPLES BY RFLP-DGGE METHOD

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JAHANI SHERAFAT S.; TAJEDDIN E.; ALEBOUYEH M.; NAZEMALHOSSEINI MOJARAD E.; MOHAMMAD ALIZADEH A.H.; SEYED MAJIDI M.R.; ZAL M.R.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2010 | Volume:2 | Issue:6 Page(s): 19-24

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Title

MOLECULAR TYPING OF E. COLI ISOLATES FROM BILE DUCT SAMPLES BY RFLP-DGGE METHOD

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Keywords

RFLP–DGGEQ3

E.COLIQ3

GALLQ2

Abstract

Background and Objective: The aim of present study is detection of the diversity of responsible bacterial isolates have identified from bile duct infections and investigation of the sensitivity of RFLP-DGGE method as an alternative method for discrimination of these isolates.Materials and Methods: 60 bile samples were collected from Taleghani hospitalized patients in Tehran. Identification of bacteria and antimicrobial susceptibility tests were done according to the standard methods. For the detection of different isolates, PCR on 16s rRNA region and RFLP-DGGE were done.Results: 16 out of 38 bacterial isolates were identified as E.COLI. The highest resistance phenotypes were belonged to Amoxicillin (100%) and Ampicillin (75%). RFLP-DGGE show ed different banding patterns, so that different isolates could be discriminated from each others, easily.Conclusion: These results showed that RFLP- DGGE on 16s rRNA PCR products could be considered as a suitable method for differentiation of bacterial isolates from clinical samples.

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APA: Copy

JAHANI SHERAFAT, S., TAJEDDIN, E., ALEBOUYEH, M., NAZEMALHOSSEINI MOJARAD, E., MOHAMMAD ALIZADEH, A.H., SEYED MAJIDI, M.R., & ZAL, M.R.. (2010). MOLECULAR TYPING OF E. COLI ISOLATES FROM BILE DUCT SAMPLES BY RFLP-DGGE METHOD. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 2(6), 19-24. SID. https://sid.ir/paper/181450/en

Vancouver: Copy

JAHANI SHERAFAT S., TAJEDDIN E., ALEBOUYEH M., NAZEMALHOSSEINI MOJARAD E., MOHAMMAD ALIZADEH A.H., SEYED MAJIDI M.R., ZAL M.R.. MOLECULAR TYPING OF E. COLI ISOLATES FROM BILE DUCT SAMPLES BY RFLP-DGGE METHOD. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2010;2(6):19-24. Available from: https://sid.ir/paper/181450/en

IEEE: Copy

S. JAHANI SHERAFAT, E. TAJEDDIN, M. ALEBOUYEH, E. NAZEMALHOSSEINI MOJARAD, A.H. MOHAMMAD ALIZADEH, M.R. SEYED MAJIDI, and M.R. ZAL, “MOLECULAR TYPING OF E. COLI ISOLATES FROM BILE DUCT SAMPLES BY RFLP-DGGE METHOD,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 2, no. 6, pp. 19–24, 2010, [Online]. Available: https://sid.ir/paper/181450/en

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