COMPARISON OF PCR-RFLP AND MICROSCOPY METHODS FOR DETECTION OF PLASMODIUM PARASITE IN ENDEMIC AREA

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SHARIFI KH.; BANDEHPOUR M.; JAMSHIDI M.; SEYED N.; NAJARI M.; ASADI SH.; KAZEMI BAHRAM

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2009 | Volume:1 | Issue:1 Page(s): 1-5

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Title

COMPARISON OF PCR-RFLP AND MICROSCOPY METHODS FOR DETECTION OF PLASMODIUM PARASITE IN ENDEMIC AREA

Author(s)

Keywords

MALARIAQ2

MICROSCOPIC METHODQ3

PCR-RFLPQ3

Abstract

Background and Objective: MALARIA is one of the most important health problems in tropical countries. The main purpose of this study was to compare microscopically and PCR-RFLP methods for diagnosis of plasmodium parasites in dubious patients.Materials and Methods: We collected 102 blood samples which 77 of them are from patients referred to Bandar Abbas hospital and 25 of them are from healthy persons which used as control group. Slide thick and thin smears were prepared and observed by microscope. Blood DNA was extract and PCR reaction was performed.Results: By using microscope methods, 35 % of samples were positive hence 75.2 % of them were PCR positive. In this study none of the samples which were reported positive by using microscope methods, were negative in PCR. For differentiation of plasmodium parasites RFLP was used which there were 12.96% P. MALARIAe, 14.8% p. falciparum, 20.7% p.vivax, and 42.69% mix of plasmodium spp were observed.Conclusion: PCR method is more sensitive than microscopically methods for MALARIA parasites detection.

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APA: Copy

SHARIFI, KH., BANDEHPOUR, M., JAMSHIDI, M., SEYED, N., NAJARI, M., ASADI, SH., & KAZEMI, BAHRAM. (2009). COMPARISON OF PCR-RFLP AND MICROSCOPY METHODS FOR DETECTION OF PLASMODIUM PARASITE IN ENDEMIC AREA. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 1(1), 1-5. SID. https://sid.ir/paper/181458/en

Vancouver: Copy

SHARIFI KH., BANDEHPOUR M., JAMSHIDI M., SEYED N., NAJARI M., ASADI SH., KAZEMI BAHRAM. COMPARISON OF PCR-RFLP AND MICROSCOPY METHODS FOR DETECTION OF PLASMODIUM PARASITE IN ENDEMIC AREA. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2009;1(1):1-5. Available from: https://sid.ir/paper/181458/en

IEEE: Copy

KH. SHARIFI, M. BANDEHPOUR, M. JAMSHIDI, N. SEYED, M. NAJARI, SH. ASADI, and BAHRAM KAZEMI, “COMPARISON OF PCR-RFLP AND MICROSCOPY METHODS FOR DETECTION OF PLASMODIUM PARASITE IN ENDEMIC AREA,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 1, no. 1, pp. 1–5, 2009, [Online]. Available: https://sid.ir/paper/181458/en

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