EVALUATION OF PHYLOGENETIC TYPING OF ESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION

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ETEBARZADEH Z.; OSHAGHI M.; AMIR MOZAFARI N.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:13 Page(s): 29-36

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Title

EVALUATION OF PHYLOGENETIC TYPING OF ESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION

Author(s)

ETEBARZADEH Z. | OSHAGHI M. | AMIR MOZAFARI N. | Issue Writer Certificate

Keywords

E.COLIQ2

PHYLOGENETIC TYPINGQ2

URINARY TRACT INFECTION (UTI)Q2

Abstract

Background and Objective: Urinary tract infections (UTIs) are one of the most common infectious diseases that E. coli is the major causative pathogen. Phylogenetic analyses have shown that E. coli strains fall into four phylogenetic groups (A, B1, B2 and D). This study aimed to define the phylogenetic groups of E.COLI isolated from urinary tract infections.Materials and Methods: This study was carried out on the patients of suspected urinary tract infection. Specific biochemical and microbial tests were used for identification of isolated bacteria. The phylogenetic groups of E.COLI strains were determined by Multiplex PCR technique of two genes (chuA and yjaA) and a DNA fragment (TSPE4.C2). After electrophorese, strains were divided in phylogenetic groups based on the presence or absence of genes and a DNA fragment.Results: Out of 100 identified E. coli, the most common identified groups were classified as B2 (65%), D (19%) and A (16%), and none of the species belonged to the B1 group. According to the findings of this study, most of the extra-intestinal pathogenic strains of E. coli isolated from urinary tract infections belonged to phylogenetic group B2.Conclusion: The results showed that the molecular PHYLOGENETIC TYPING method based on PCR of genes and a DNA fragment is a simple and rapid method in typing of E. coli strains. Also this technique uses for understanding about pathogenesis of infections, hospital infections control and epidemiological studies.

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APA: Copy

ETEBARZADEH, Z., OSHAGHI, M., & AMIR MOZAFARI, N.. (2012). EVALUATION OF PHYLOGENETIC TYPING OF ESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(13), 29-36. SID. https://sid.ir/paper/181459/en

Vancouver: Copy

ETEBARZADEH Z., OSHAGHI M., AMIR MOZAFARI N.. EVALUATION OF PHYLOGENETIC TYPING OF ESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(13):29-36. Available from: https://sid.ir/paper/181459/en

IEEE: Copy

Z. ETEBARZADEH, M. OSHAGHI, and N. AMIR MOZAFARI, “EVALUATION OF PHYLOGENETIC TYPING OF ESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 13, pp. 29–36, 2012, [Online]. Available: https://sid.ir/paper/181459/en

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