STUDY OF PXO PLASMID PREVALENCE IN NON PATHOGENIC BACILLUS AND RECONSIDERING IN CLASSIFYING BACILLUS WITH PCR METHOD

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MORADI H.A.; NOROUZI J.; SHAHHOSSEINY M.H.; JAVADI G.R.; SHAHCHERAGHI H.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:13 Page(s): 21-28

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Title

STUDY OF PXO PLASMID PREVALENCE IN NON PATHOGENIC BACILLUS AND RECONSIDERING IN CLASSIFYING BACILLUS WITH PCR METHOD

Author(s)

Keywords

PCRQ2

BACILLUSQ2

PXO1 PLASMIDQ2

PXO2 PLASMIDQ2

Abstract

Background and Objective: Anthrax disease is caused by BACILLUS anthracis. BACILLUS anthracis has PXO1 PLASMID and PXO2 PLASMID. PXO1 PLASMID is responsible for toxin production and the PXO2 PLASMID is synthesized capsule. The purpose of this study has been examined for presence the gene Pxo in BACILLUS subtilis, BACILLUS cereus and BACILLUS thuringiensis.Materials and Methods: In this cross sectional, 65 soil samples collected from across the country and biochemical tests were performed then The plasmid was extracted. Using specific primers Toxin gene in PXO1 PLASMID and gene capsule in PXO2 PLASMID Examined.Results: From 65 Soil samples were collected 31Bacillus were isolated. They were 19 BACILLUS cereus, 12 BACILLUS subtilis and also7 BACILLUS donated that Includes 2 BACILLUS cereus and 2 BACILLUS subtilis and were 3 BACILLUS thuringiensis. From whole BACILLUS isolated Only 13 BACILLUS cereus has bond toxin encoding gene (pXO1). Band coding capsule gene (pXO2) has not in the case of BACILLUS.Conclusion: This study showed that PXO1 PLASMID has been transferred from BACILLUS anthracis to the BACILLUS cereus in Iran but none of the items had been done.

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APA: Copy

MORADI, H.A., NOROUZI, J., SHAHHOSSEINY, M.H., JAVADI, G.R., & SHAHCHERAGHI, H.. (2012). STUDY OF PXO PLASMID PREVALENCE IN NON PATHOGENIC BACILLUS AND RECONSIDERING IN CLASSIFYING BACILLUS WITH PCR METHOD. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(13), 21-28. SID. https://sid.ir/paper/181464/en

Vancouver: Copy

MORADI H.A., NOROUZI J., SHAHHOSSEINY M.H., JAVADI G.R., SHAHCHERAGHI H.. STUDY OF PXO PLASMID PREVALENCE IN NON PATHOGENIC BACILLUS AND RECONSIDERING IN CLASSIFYING BACILLUS WITH PCR METHOD. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(13):21-28. Available from: https://sid.ir/paper/181464/en

IEEE: Copy

H.A. MORADI, J. NOROUZI, M.H. SHAHHOSSEINY, G.R. JAVADI, and H. SHAHCHERAGHI, “STUDY OF PXO PLASMID PREVALENCE IN NON PATHOGENIC BACILLUS AND RECONSIDERING IN CLASSIFYING BACILLUS WITH PCR METHOD,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 13, pp. 21–28, 2012, [Online]. Available: https://sid.ir/paper/181464/en

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