COMPARISON OF ALLERGIC REACTIONS OF TRICHOPHYTON RUBRUM AND MICROSPORUM CANISIN GUINEA PIG AND PLANNING OF TRI R4 GEN SPECIFIC PRIMER

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BAHRAMZADEH DAII M.; BAYAT M.; GHAFFARIFAR F.; ROUDBAR MOHAMMADI S.; ENTEZARI M.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2011 | Volume:2 | Issue:7 Page(s): 9-16

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Title

COMPARISON OF ALLERGIC REACTIONS OF TRICHOPHYTON RUBRUM AND MICROSPORUM CANISIN GUINEA PIG AND PLANNING OF TRI R4 GEN SPECIFIC PRIMER

Author(s)

Keywords

TRICHOPHYTON RUBRUMQ3

MICROSPORUM CANISQ3

PCRQ2

ALLERGYQ2

TRI R 4Q3

Abstract

Background and Objective: TRICHOPHYTON RUBRUM is a wild spread fungi which cause the Dermatophytosis and approximately 80-93% of chronic or recurrent/recurdescent dermatophyte infections are created by TRICHOPHYTON RUBRUM. Tricophyton proteins are members of different enzymatic families and dual role of these proteins in fungal pathogenesis as well as their allergenic behavior. Among TRICHOPHYTON RUBRUM allergens, Tri r 2 and TRI R 4 can be mentioned. The present research aims to investigate the allergenic behavior of TRICHOPHYTON RUBRUM and MICROSPORUM CANIS.Materials and Methods: Initially, the fungi TRICHOPHYTON RUBRUM was cultured on PDA medium.Their conidia were subsequently gathered by sterilized PBS. DNA was extracted and PCR experiment was fulfilled using the specific primers Tri r4. Additionally, Guinea pig was used to study the allergenic behavior of the fungi TRICHOPHYTON RUBRUM and MICROSPORUM CANIS. To confirm that the fungi were allergenic, total IgE antibody was assayed by ELISA method. Also, in CBC test performed on Guinea pig blood samples, percentage of blood cells especially eosinophils were evaluated.Results: Conidia were confirmed by using PCR test for Tri r4. Results of allergenic behavior indicated that more IgE antibody, also neutrophil and eosinophil were produced in the Guinea pig in which TRICHOPHYTON RUBRUMwas inoculated and has a significant different compared to control group, whereas no significant different was observed in comparison with control group in the Guinea pig in which MICROSPORUM CANIS was inoculated.Conclusion: The present research indicates direct effects of allergens in enhancing titer of IgE antibody.

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APA: Copy

BAHRAMZADEH DAII, M., BAYAT, M., GHAFFARIFAR, F., ROUDBAR MOHAMMADI, S., & ENTEZARI, M.. (2011). COMPARISON OF ALLERGIC REACTIONS OF TRICHOPHYTON RUBRUM AND MICROSPORUM CANISIN GUINEA PIG AND PLANNING OF TRI R4 GEN SPECIFIC PRIMER. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 2(7), 9-16. SID. https://sid.ir/paper/181489/en

Vancouver: Copy

BAHRAMZADEH DAII M., BAYAT M., GHAFFARIFAR F., ROUDBAR MOHAMMADI S., ENTEZARI M.. COMPARISON OF ALLERGIC REACTIONS OF TRICHOPHYTON RUBRUM AND MICROSPORUM CANISIN GUINEA PIG AND PLANNING OF TRI R4 GEN SPECIFIC PRIMER. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;2(7):9-16. Available from: https://sid.ir/paper/181489/en

IEEE: Copy

M. BAHRAMZADEH DAII, M. BAYAT, F. GHAFFARIFAR, S. ROUDBAR MOHAMMADI, and M. ENTEZARI, “COMPARISON OF ALLERGIC REACTIONS OF TRICHOPHYTON RUBRUM AND MICROSPORUM CANISIN GUINEA PIG AND PLANNING OF TRI R4 GEN SPECIFIC PRIMER,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 2, no. 7, pp. 9–16, 2011, [Online]. Available: https://sid.ir/paper/181489/en

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