STUDY THE ROLE OF ISOLATED BACTERIA FROM OIL CONTAMINATED SOIL IN BIOREMEDIATION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

VAHID S.; AKHAVANSEPAHI A.; NOUHI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN) Year:2008 | Volume:3 | Issue:1 Page(s): 1-15

Download Full-Text

Persian Verion

View:

3,058

Download:

Cites:

Information Journal Paper

Title

STUDY THE ROLE OF ISOLATED BACTERIA FROM OIL CONTAMINATED SOIL IN BIOREMEDIATION

Author(s)

VAHID S. | AKHAVANSEPAHI A. | NOUHI A. | Issue Writer Certificate

Keywords

BIOREMEDIATIONQ2

CRUDE OILQ2

BIOSURFACTANTQ2

Abstract

The main purpose in this research is, collection and recognition bacteria that use oil as the source of energy and carbon, degrade it to recyclable material, find biosurfactatnt producing bacteria, know their applied potential in oil industry, remove oily contaminated elements and clean the nature soil and water environment. For separating bacteria from contaminated soil and water, was cultured with serial dilution method in Plate Count Agar medium, then the number of bacteria and the rate of isolation was increased by culturing the bacteria in enrichment medium (MS1) that was suitable for oil degrading bacteria, then they was cultured in synthetic medium that contains Agar+1% sterile CRUDE OIL as the source of carbon and energy, and 40 strains was selected. Then blood agar medium was used for haemolytic activity and MSS+1%Yeast Extract medium for measurement of surface tension, as the point of early separating and separating BIOSURFACTANT producing bacteria, respectively. 12 different strains was collected with beta haemolitic activities, at last 3 strains that could reduce surface tension less than 40 mN/m was chosen. By using additional tests such as, toxicity test of CRUDE OIL by using of MS2 medium, measurement of growth and produce BIOSURFACTANT in different salt concentration, BIOSURFACTANT producing from non-oily sources and measuring emulsifying activity in Cooper medium, one strain was selected, as the best BIOSURFACTANT producing strain that has highest results in experiments. Selected strain was a Gram-negative Coccobacillus that can produce BIOSURFACTANT in wide range of pH (2-14), and using glucose and paraffin as the source of carbon and reduce surface tension less than 40 mN/m. Produced BIOSURFACTANT was recognized as glycolipids in nature that was stable during exposure to high salinity (up to10% NaCl) and elevated temperatures (100-120oC for 15 minutes) by TLC method. At last by 16S rRNA sequencing, the real bacterial genus and strain was recognized as: Pseudomonas aeruginosa.

Cites

No record.

References

No record.

Cite

APA: Copy

VAHID, S., AKHAVANSEPAHI, A., & NOUHI, A.. (2008). STUDY THE ROLE OF ISOLATED BACTERIA FROM OIL CONTAMINATED SOIL IN BIOREMEDIATION. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), 3(1), 1-15. SID. https://sid.ir/paper/183701/en

Vancouver: Copy

VAHID S., AKHAVANSEPAHI A., NOUHI A.. STUDY THE ROLE OF ISOLATED BACTERIA FROM OIL CONTAMINATED SOIL IN BIOREMEDIATION. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN)[Internet]. 2008;3(1):1-15. Available from: https://sid.ir/paper/183701/en

IEEE: Copy

S. VAHID, A. AKHAVANSEPAHI, and A. NOUHI, “STUDY THE ROLE OF ISOLATED BACTERIA FROM OIL CONTAMINATED SOIL IN BIOREMEDIATION,” BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), vol. 3, no. 1, pp. 1–15, 2008, [Online]. Available: https://sid.ir/paper/183701/en

Related Journal Papers