DETECTION OF FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES BY RT-PCR

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GHOREYSHI S.A.; DALIRI MORTEZA; HAJIAN T.; BANOUEI M.M.; ALVANDI A.R.

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Journal: Year:2002 | Volume:14 | Issue:4 (53 IN ANIMAL & FISHRIES SCIENCES) Page(s): 10-12

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Title

DETECTION OF FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES BY RT-PCR

Author(s)

Keywords

RT-PCRQ3

FMDVQ2

DETECTIONQ3

Abstract

In order to utilize a reliable, fast and sensitive test for DETECTION of Foot-and-Mouth disease virus (FMDV) in clinical samples, a Reverse - Transcriptase Polymerase Chain Reaction (RT -PCR) method was optimized. This RT-PCR can detect FMD viral RNA in samples regardless of their serotypes. Four vaccine viral strains of types A (two isolates), 0 and Asia 1 were tested and a 131 bp pcr product from 28 region of FMDV genome was amplified. Thirty-seven clinical samples were also tested. The specificity of results was confirmed by direct sequencing of PCR products. This rapid molecular diagnostic method, which is sensitive and specific, can detect FMDV in clinical samples in less than eight hours. Therefore, it would be valuable for early DETECTION of suspected FMDV outbreaks.

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References

بیماری تب برفکی (FMD) و وضعیت آن در ایران

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APA: Copy

GHOREYSHI, S.A., DALIRI, MORTEZA, HAJIAN, T., BANOUEI, M.M., & ALVANDI, A.R.. (2002). DETECTION OF FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES BY RT-PCR. PAJOUHESH-VA-SAZANDEGI, 14(4 (53 IN ANIMAL & FISHRIES SCIENCES)), 10-12. SID. https://sid.ir/paper/18739/en

Vancouver: Copy

GHOREYSHI S.A., DALIRI MORTEZA, HAJIAN T., BANOUEI M.M., ALVANDI A.R.. DETECTION OF FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES BY RT-PCR. PAJOUHESH-VA-SAZANDEGI[Internet]. 2002;14(4 (53 IN ANIMAL & FISHRIES SCIENCES)):10-12. Available from: https://sid.ir/paper/18739/en

IEEE: Copy

S.A. GHOREYSHI, MORTEZA DALIRI, T. HAJIAN, M.M. BANOUEI, and A.R. ALVANDI, “DETECTION OF FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES BY RT-PCR,” PAJOUHESH-VA-SAZANDEGI, vol. 14, no. 4 (53 IN ANIMAL & FISHRIES SCIENCES), pp. 10–12, 2002, [Online]. Available: https://sid.ir/paper/18739/en

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