APOPTOSIS WILL BE INCREASED AFTER 2H INCUBATION OF HUMAN SPERM AT 37OC

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NABI A.; KHALILI M.A.; HALVAEI I.; ANBARI F.

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Journal: JOURNAL OF CELL & TISSUE Year:2014 | Volume:4 | Issue:4 Page(s): 415-423

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Title

APOPTOSIS WILL BE INCREASED AFTER 2H INCUBATION OF HUMAN SPERM AT 37OC

Author(s)

NABI A. | KHALILI M.A. | HALVAEI I. | ANBARI F. | Issue Writer Certificate

Keywords

SPERMATOZOAQ2

DNA FRAGMENTATIONQ2

TUNELQ2

Abstract

Aim: The main goal was to evaluate the impact of different incubation time intervals on human sperm DNA status using terminal deoxyribonucleotidyl transferase–mediated dUTP nick-end labeling (TUNEL) test.Material and methods: This prospective study involved 21 normozoospermic specimens. After direct swim-up, sperm cells were incubated at 37oC and DNA damage was evaluated at different time intervals (0, 1, 2 and 3 h). After slide fixation with methanol 100% for 4 minutes and rinse in phosphate-buffered solution (PBS), TUNEL was added to each slide and incubated for 60 minute in dark. Eosin-Nigrosin and Papanicolaou staining protocols were applied in order to assess sperm viability and morphology, respectively.Results: Sperm viability and normal morphology was improved after sperm processing (100%, 72.3% respectively, p<0.0001). The rate of DNA damage was significantly higher after 2h compared to 0h (9.19±0.8% Vs 4.9±0.9%, respectively, p=0.008). Also there was significant difference in abnormal sperm DNA between 3h and 1h (10.95±0.7% Vs 7.1±1%, respectively, p=0.020).Conclusion: Incubation of prepared normozoospermic samples at 37oC more than 2 h may be associated with sperm DNA FRAGMENTATION. Therefore, it seems that incubation of human SPERMATOZOA at 37oC should be limited up to 2h prior to use in ART clinics.

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APA: Copy

NABI, A., KHALILI, M.A., HALVAEI, I., & ANBARI, F.. (2014). APOPTOSIS WILL BE INCREASED AFTER 2H INCUBATION OF HUMAN SPERM AT 37^OC. JOURNAL OF CELL & TISSUE, 4(4), 415-423. SID. https://sid.ir/paper/188927/en

Vancouver: Copy

NABI A., KHALILI M.A., HALVAEI I., ANBARI F.. APOPTOSIS WILL BE INCREASED AFTER 2H INCUBATION OF HUMAN SPERM AT 37^OC. JOURNAL OF CELL & TISSUE[Internet]. 2014;4(4):415-423. Available from: https://sid.ir/paper/188927/en

IEEE: Copy

A. NABI, M.A. KHALILI, I. HALVAEI, and F. ANBARI, “APOPTOSIS WILL BE INCREASED AFTER 2H INCUBATION OF HUMAN SPERM AT 37^OC,” JOURNAL OF CELL & TISSUE, vol. 4, no. 4, pp. 415–423, 2014, [Online]. Available: https://sid.ir/paper/188927/en

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