INVESTIGATING THE EFFECT OF CIRSIUM VULGARE HYDROETHANOLIC EXTRACT ON NEURAL STEM CELLS PROLIFERATION

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ABDANIPOUR A.R.; KHATAMI S.M.; SAGHA M.; SALIMI NANEHKARAN F.; NAGHIZADEH D.; BONABI R.

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Journal: JOURNAL OF CELL & TISSUE Year:2015 | Volume:5 | Issue:4 Page(s): 385-391

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Title

INVESTIGATING THE EFFECT OF CIRSIUM VULGARE HYDROETHANOLIC EXTRACT ON NEURAL STEM CELLS PROLIFERATION

Author(s)

Keywords

CELL PROLIFERATIONQ2

CIRSIUM VULGAREQ2

NEURAL STEM CELLSQ2

Abstract

Aim: In vitro investigation of hydroethanolic extraction of CIRSIUM VULGARE on growth rates of neonate rat NEURAL STEM CELLS.Material and Methods: NEURAL STEM CELLS were isolated from hippocampus of neonatal rat brain. To determine optimal concentration of CIRSIUM VULGARE extraction, isolated NEURAL STEM CELLS were treated with 200, 400, 600, 800 and 1000 mg/ml for 48 h and then cells proliferation rate were evaluated by MTT assay. In addition Sox2 mRNA expression in NEURAL STEM CELLS was evaluated using quantitative real-time PCR.Results: The results of this study showed that the CIRSIUM VULGARE extract caused significant increase in cell proliferative activity and Sox2 mRNA expression when compared with the control group.Conclusion: with respect to the effect of CIRSIUM VULGARE extract on the proliferation rate of NEURAL STEM CELLS, the use of hydroethanolic extraction of CIRSIUM VULGARE can be used for clinical purposes to treat some of the neurodegenerative disorders such as ischemic stroke and spinal cord injury.

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APA: Copy

ABDANIPOUR, A.R., KHATAMI, S.M., SAGHA, M., SALIMI NANEHKARAN, F., NAGHIZADEH, D., & BONABI, R.. (2015). INVESTIGATING THE EFFECT OF CIRSIUM VULGARE HYDROETHANOLIC EXTRACT ON NEURAL STEM CELLS PROLIFERATION. JOURNAL OF CELL & TISSUE, 5(4), 385-391. SID. https://sid.ir/paper/188937/en

Vancouver: Copy

ABDANIPOUR A.R., KHATAMI S.M., SAGHA M., SALIMI NANEHKARAN F., NAGHIZADEH D., BONABI R.. INVESTIGATING THE EFFECT OF CIRSIUM VULGARE HYDROETHANOLIC EXTRACT ON NEURAL STEM CELLS PROLIFERATION. JOURNAL OF CELL & TISSUE[Internet]. 2015;5(4):385-391. Available from: https://sid.ir/paper/188937/en

IEEE: Copy

A.R. ABDANIPOUR, S.M. KHATAMI, M. SAGHA, F. SALIMI NANEHKARAN, D. NAGHIZADEH, and R. BONABI, “INVESTIGATING THE EFFECT OF CIRSIUM VULGARE HYDROETHANOLIC EXTRACT ON NEURAL STEM CELLS PROLIFERATION,” JOURNAL OF CELL & TISSUE, vol. 5, no. 4, pp. 385–391, 2015, [Online]. Available: https://sid.ir/paper/188937/en

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