COMPARISON OF DBAT GENE SEQUENCE, IN NUCLEOTIDE AND AMINO ACID LEVEL, IN YEW SPECIES AND FUNGAL ENDOPHYTES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SEIFI M.; NAZERI S.; SOLTANI J.; CHEHREGANI RAD A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CELL & TISSUE Year:2013 | Volume:4 | Issue:2 Page(s): 197-203

Download Full-Text

Persian Verion

View:

1,064

Download:

Cites:

Information Journal Paper

Title

COMPARISON OF DBAT GENE SEQUENCE, IN NUCLEOTIDE AND AMINO ACID LEVEL, IN YEW SPECIES AND FUNGAL ENDOPHYTES

Author(s)

SEIFI M. | NAZERI S. | SOLTANI J. | CHEHREGANI RAD A. | Issue Writer Certificate

Keywords

TAXOLQ2

FUNGIQ1

TAXUS BACCATAQ3

DBAT GENEQ2

Abstract

Aim: The aim of this study was investigation of the presence DBAT GENE, a key gene in the biosynthetic pathway of TAXOL, in yew (Taxus baccata) and its TAXOL producing endophytic fungus and also assessment the similarity of these sequences with sequences available in GenBank.Material and Methods: In this study, endophytic FUNGI were isolated from the bark of TAXUS BACCATA and purified, using hyphal tip method. Genomic DNA of yew tree and a TAXOL producing fungus (T9 isolate) was extracted. Using polymerase chain reaction, presence of DBAT GENE was investigated. After sequencing, similarity of sequences obtained from yew and T9 isolate with other taxus species and endophyticn FUNGI sequences, available in GenBank, were compared.Results: Using specific primers, a 125 bp fragment of DBAT GENE from TAXUS BACCATA and T9 isolate was amplified. Nucleotide and amino acid sequences alignment showed that theses sequences had a high similarity (over 98%) in fungus and host plant. Alignment results of these sequences with other species of yew trees and endophytic FUNGI sequences also showed very high similarity (98-100%). Besides, relatively high similarity between the nucleotides and amino acid sequences of DBAT and other acyl/aroyltransferases enzymes involved in TAXOL biosynthesis were observed.Conclusion: Based on the results, the presence of DBAT GENE in TAXOL producing endophytic fungus and also very high homology of its sequence, in nucleotide and amino acid levels, with host plant (Taxus baccata) and other yew species and fungal endophytes were proved.

Cites

No record.

References

No record.

Cite

APA: Copy

SEIFI, M., NAZERI, S., SOLTANI, J., & CHEHREGANI RAD, A.. (2013). COMPARISON OF DBAT GENE SEQUENCE, IN NUCLEOTIDE AND AMINO ACID LEVEL, IN YEW SPECIES AND FUNGAL ENDOPHYTES. JOURNAL OF CELL & TISSUE, 4(2), 197-203. SID. https://sid.ir/paper/188959/en

Vancouver: Copy

SEIFI M., NAZERI S., SOLTANI J., CHEHREGANI RAD A.. COMPARISON OF DBAT GENE SEQUENCE, IN NUCLEOTIDE AND AMINO ACID LEVEL, IN YEW SPECIES AND FUNGAL ENDOPHYTES. JOURNAL OF CELL & TISSUE[Internet]. 2013;4(2):197-203. Available from: https://sid.ir/paper/188959/en

IEEE: Copy

M. SEIFI, S. NAZERI, J. SOLTANI, and A. CHEHREGANI RAD, “COMPARISON OF DBAT GENE SEQUENCE, IN NUCLEOTIDE AND AMINO ACID LEVEL, IN YEW SPECIES AND FUNGAL ENDOPHYTES,” JOURNAL OF CELL & TISSUE, vol. 4, no. 2, pp. 197–203, 2013, [Online]. Available: https://sid.ir/paper/188959/en

Related Journal Papers