THE EFFECT OF VITRIFICATION ON MATURATION AND ULTRASTRUCTURE OF IN-VITRO MATURED HUMAN OOCYTES

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SHAHEDI A.; HOSSEINI A.

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Journal: JOURNAL OF CELL & TISSUE Year:2016 | Volume:7 | Issue:1 Page(s): 33-44

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Title

THE EFFECT OF VITRIFICATION ON MATURATION AND ULTRASTRUCTURE OF IN-VITRO MATURED HUMAN OOCYTES

Author(s)

SHAHEDI A. | HOSSEINI A. | Issue Writer Certificate

Keywords

HUMAN OOCYTEQ1

VITRIFICATIONQ2

IN VITRO MATURATIONQ1

ULTRASTRUCTUREQ2

Abstract

Aim: The aim of this study was to describe the possible effects of VITRIFICATION on maturation rate and ultrastructural morphology of in-vitromatured HUMAN OOCYTEs.Material and Methods: A total of 292 immature Germinal Vesicle (GV) and Metaphase I (MI) oocytes obtained from infertile patients were allocated into two groups: (i) GV oocytes (n=145), (ii) MI oocytes (n=147). Oocytes were first vitrified and then matured in-vitro. Supernumerary fresh invivo matured oocytes (n=10) were used as control. in-vitromaturation media was Ham’s F10 supplemented with FSH+LH and human follicular fluid. After 36h of incubation, the oocytes were investigated for nuclear maturation and ultrastructural changes.Results: The rate of survival and degeneration was significantly higher in Metaphase I than GV group.But, Oocyte maturation rates were not significant between groups (P<0.06). In addition, the rate of oocyte arrest was significantly higher in MI oocytes in comparisson with GV oocytes.Ultrastructurally; a drastic reduction was observed in amount of cortical granules at the cortex of vitrified-warmed GV and MI oocytes, In addition, Transmission Electron Microscopy (TEM) revealed vacuoles and small mitochondria-Smooth Endoplasmic Reticulum aggregates in the ooplasm of oocytes.conclusion: Freeze/thawing procedures are associated with ultrastructural alterations in specific oocyte microdomains, presumably related to the reduced competence of cryopreserved oocytes to maturation.

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APA: Copy

SHAHEDI, A., & HOSSEINI, A.. (2016). THE EFFECT OF VITRIFICATION ON MATURATION AND ULTRASTRUCTURE OF IN-VITRO MATURED HUMAN OOCYTES. JOURNAL OF CELL & TISSUE, 7(1), 33-44. SID. https://sid.ir/paper/188969/en

Vancouver: Copy

SHAHEDI A., HOSSEINI A.. THE EFFECT OF VITRIFICATION ON MATURATION AND ULTRASTRUCTURE OF IN-VITRO MATURED HUMAN OOCYTES. JOURNAL OF CELL & TISSUE[Internet]. 2016;7(1):33-44. Available from: https://sid.ir/paper/188969/en

IEEE: Copy

A. SHAHEDI, and A. HOSSEINI, “THE EFFECT OF VITRIFICATION ON MATURATION AND ULTRASTRUCTURE OF IN-VITRO MATURED HUMAN OOCYTES,” JOURNAL OF CELL & TISSUE, vol. 7, no. 1, pp. 33–44, 2016, [Online]. Available: https://sid.ir/paper/188969/en

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