EVALUATION OF GROWTH AND DIFFERENTIATION OF WHARTON'S JELLY-DERIVED MESENCHYMAL STEM CELLS TO OSTEOBLAST ON POLYCAPROLACTONE COMPOSITE SCAFFOLDS (PCL)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GAZIJAHANI SH.; YAGHOUBI H.; ASADI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CELL & TISSUE Year:2016 | Volume:7 | Issue:2 Page(s): 179-190

Download Full-Text

Persian Verion

View:

732

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF GROWTH AND DIFFERENTIATION OF WHARTON'S JELLY-DERIVED MESENCHYMAL STEM CELLS TO OSTEOBLAST ON POLYCAPROLACTONE COMPOSITE SCAFFOLDS (PCL)

Author(s)

GAZIJAHANI SH. | YAGHOUBI H. | ASADI A. | Issue Writer Certificate

Keywords

CELL DIFFERENTIATIONQ2

POLYCAPROLACTONEQ1

STEMS CELLQ2

TISSUE ENGINEERINGQ2

UMBILICAL CORDQ1

Abstract

Aim: The aim of this study was to evaluate the biocompatibility and differentiation of human UMBILICAL CORD mesenchymal stem cells to osteoblast on prepared POLYCAPROLACTONE scaffolds by electrospinning method, which is located under the surface modification by oxygen plasma.Material and methods: After isolation of mesenchymal stem cells from human UMBILICAL CORD, flow cytometry analysis was performed. Biocompatibility of scaffold was examined using MTT assay.Cells morphology and their adhesion characteristics on the scaffold surface were studied using Scanning Electron Microscopic (SEM) images. Also biodegradability of scaffold calculated using weight loss method. Finally, for study the differentiation of cells on the scaffold surface, osteogenic differentiation medium was used and differentiation was performed by red alizarin staining and RTPCR.Results: Results showed that the cells not only had more suitable connection and reproduction abilities on the scaffold, but also were morphologically in a natural condition. Polycaprolacton scaffold is enjoyment of high biocompatibility and its biodegradability up to 15th day done with more rates in comparison with 15 next days. Also red alizarin staining and RT-PCR results showed high rate CELL DIFFERENTIATION on POLYCAPROLACTONE scaffold.Conclusion: The obtained results of this research suggested that POLYCAPROLACTONE scaffolds have potential usage as biocompatible biomaterials in TISSUE ENGINEERING and osteogenic differentiation applications.

Cites

No record.

References

No record.

Cite

APA: Copy

GAZIJAHANI, SH., YAGHOUBI, H., & ASADI, A.. (2016). EVALUATION OF GROWTH AND DIFFERENTIATION OF WHARTON'S JELLY-DERIVED MESENCHYMAL STEM CELLS TO OSTEOBLAST ON POLYCAPROLACTONE COMPOSITE SCAFFOLDS (PCL). JOURNAL OF CELL & TISSUE, 7(2 ), 179-190. SID. https://sid.ir/paper/189003/en

Vancouver: Copy

GAZIJAHANI SH., YAGHOUBI H., ASADI A.. EVALUATION OF GROWTH AND DIFFERENTIATION OF WHARTON'S JELLY-DERIVED MESENCHYMAL STEM CELLS TO OSTEOBLAST ON POLYCAPROLACTONE COMPOSITE SCAFFOLDS (PCL). JOURNAL OF CELL & TISSUE[Internet]. 2016;7(2 ):179-190. Available from: https://sid.ir/paper/189003/en

IEEE: Copy

SH. GAZIJAHANI, H. YAGHOUBI, and A. ASADI, “EVALUATION OF GROWTH AND DIFFERENTIATION OF WHARTON'S JELLY-DERIVED MESENCHYMAL STEM CELLS TO OSTEOBLAST ON POLYCAPROLACTONE COMPOSITE SCAFFOLDS (PCL),” JOURNAL OF CELL & TISSUE, vol. 7, no. 2 , pp. 179–190, 2016, [Online]. Available: https://sid.ir/paper/189003/en

Related Journal Papers