EFFECT OF EMBRYONIC CEREBROSPINAL FLUID ON PROLIFERATION AND SELF-RENEWAL OF WISTAR RAT NEUROPROGENITOR CELLS

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YARI S.; PARIVAR K.; NABIUNI M.; KERAMATIPOUR M.

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Journal: JOURNAL OF CELL & TISSUE Year:2013 | Volume:4 | Issue:3 Page(s): 305-311

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Title

EFFECT OF EMBRYONIC CEREBROSPINAL FLUID ON PROLIFERATION AND SELF-RENEWAL OF WISTAR RAT NEUROPROGENITOR CELLS

Author(s)

YARI S. | PARIVAR K. | NABIUNI M. | KERAMATIPOUR M. | Issue Writer Certificate

Keywords

EMBRYONIC CEREBROSPINAL FLUIDQ2

NEUROSPHEREQ2

PROLIFERATIONQ2

Abstract

Aim: During recent years many studies have suggested some developmental roles for EMBRYONIC CEREBROSPINAL FLUID. Here we examined the effect of EMBRYONIC CEREBROSPINAL FLUID on PROLIFERATION and self-renewal of embryonic ventricular zone derived NEUROSPHERE.Material and Methods: Cortex from 15.5 day old embryonic Wistar rat dissected out and enzymaticaly dissociated to form single cell suspension. Cell suspension seeded in DMEM/F12 medium supplemented with N2 and mitogenes (10ng.ml-1 EGF and 20ng.ml-1 bFGF). The CSF-treated culture from different embryonic ages (E16, E18, and E20) in 10/100 ratio (v/v) was considered as experimental groups. NEUROSPHEREs number was counted for PROLIFERATION assay and cell viability evaluated with MTT assay. The data were analyzed with One-way ANOVA with turkey’s post hoc test.Results: EMBRYONIC CEREBROSPINAL FLUID (e-CSF) enhanced NEUROSPHERE number. Also, EMBRYONIC CEREBROSPINAL FLUID (e-CSF) enhanced cell viability and growth.Conclusion: EMBRYONIC CEREBROSPINAL FLUID (e-CSF) enhanced PROLIFERATION and viability of neural progenitor cells in age dependent manner. Moreover, this fluid play important role in establishment of neuroprogenitor cells self-renewal.

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APA: Copy

YARI, S., PARIVAR, K., NABIUNI, M., & KERAMATIPOUR, M.. (2013). EFFECT OF EMBRYONIC CEREBROSPINAL FLUID ON PROLIFERATION AND SELF-RENEWAL OF WISTAR RAT NEUROPROGENITOR CELLS. JOURNAL OF CELL & TISSUE, 4(3), 305-311. SID. https://sid.ir/paper/189023/en

Vancouver: Copy

YARI S., PARIVAR K., NABIUNI M., KERAMATIPOUR M.. EFFECT OF EMBRYONIC CEREBROSPINAL FLUID ON PROLIFERATION AND SELF-RENEWAL OF WISTAR RAT NEUROPROGENITOR CELLS. JOURNAL OF CELL & TISSUE[Internet]. 2013;4(3):305-311. Available from: https://sid.ir/paper/189023/en

IEEE: Copy

S. YARI, K. PARIVAR, M. NABIUNI, and M. KERAMATIPOUR, “EFFECT OF EMBRYONIC CEREBROSPINAL FLUID ON PROLIFERATION AND SELF-RENEWAL OF WISTAR RAT NEUROPROGENITOR CELLS,” JOURNAL OF CELL & TISSUE, vol. 4, no. 3, pp. 305–311, 2013, [Online]. Available: https://sid.ir/paper/189023/en

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