ISOLATION, CULTURE AND CHARACTERIZATION OF ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS (ADMSCS) BY EXPLANT-ENZYMATIC METHODS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHAMMADI M.H.; NABIUNI M.; PARIVAR K.; YARI S.; SAHEBI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CELL & TISSUE Year:2018 | Volume:8 | Issue:4 Page(s): 303-313

Download Full-Text

Persian Verion

View:

1,217

Download:

Cites:

Information Journal Paper

Title

ISOLATION, CULTURE AND CHARACTERIZATION OF ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS (ADMSCS) BY EXPLANT-ENZYMATIC METHODS

Author(s)

Keywords

MESENCHYMAL STEM CELLSQ2

ADIPOSE TISSUEQ1

EXPLANTQ1

RATQ2

Abstract

Aim: In this study, we use combination of enzymatic and EXPLANT methods, for isolation and culture of ADMSCs.Materials and Method: ADIPOSE TISSUEs dissected out from the abdominal region of male Wistar RATs.Tissues were minced into small pieces (1-2 mm), and then treated with trypsin-EDTA 0.25% for 30 minutes at 37oC in shaker-incubator. Enzymatic treated tissues were centrifuged and floating parts were cultured. Statistical analysis of the cells number was investigated using GraphPad Prismv6 software.Results: Results showed that ADMSCs isolated with our methods have growth characteristics similar to conventional methods. ADMSCs were maintained up to 10th passage and exhibited a homogeneous population in terms of appearance and morphology. We demonstrated that ADMSCs by these combination methods have mesenchymal characteristic markers (positive for CD90, CD44, CD73, CD105 and negative for CD35, CD45, CD11b).Conclusion: In the current study, we showed that the combination of enzymatic and EXPLANT methods creates a simple, inexpensive and reproducible method for isolation and culture of ADMSCs.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHAMMADI, M.H., NABIUNI, M., PARIVAR, K., YARI, S., & SAHEBI, A.. (2018). ISOLATION, CULTURE AND CHARACTERIZATION OF ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS (ADMSCS) BY EXPLANT-ENZYMATIC METHODS. JOURNAL OF CELL & TISSUE, 8(4 ), 303-313. SID. https://sid.ir/paper/189035/en

Vancouver: Copy

MOHAMMADI M.H., NABIUNI M., PARIVAR K., YARI S., SAHEBI A.. ISOLATION, CULTURE AND CHARACTERIZATION OF ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS (ADMSCS) BY EXPLANT-ENZYMATIC METHODS. JOURNAL OF CELL & TISSUE[Internet]. 2018;8(4 ):303-313. Available from: https://sid.ir/paper/189035/en

IEEE: Copy

M.H. MOHAMMADI, M. NABIUNI, K. PARIVAR, S. YARI, and A. SAHEBI, “ISOLATION, CULTURE AND CHARACTERIZATION OF ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS (ADMSCS) BY EXPLANT-ENZYMATIC METHODS,” JOURNAL OF CELL & TISSUE, vol. 8, no. 4 , pp. 303–313, 2018, [Online]. Available: https://sid.ir/paper/189035/en

Related Journal Papers