CYTOTOXICITY AND APOPTOTIC EFFECT OF OXALIPLATIN ON COLON CANCER CELL LINE (HT29) AND ANALYSIS OF CASPASE 3 AND CASPASE 9 GENE EXPRESSION USING REAL TIME PCR METHOD

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Yonesi B.; MIRZAIE A.; ALIASGARI E.

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Journal: JOURNAL OF CELL & TISSUE Year:2018 | Volume:8 | Issue:4 Page(s): 364-373

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Information Journal Paper

Title

CYTOTOXICITY AND APOPTOTIC EFFECT OF OXALIPLATIN ON COLON CANCER CELL LINE (HT29) AND ANALYSIS OF CASPASE 3 AND CASPASE 9 GENE EXPRESSION USING REAL TIME PCR METHOD

Author(s)

Yonesi B. | MIRZAIE A. | ALIASGARI E. | Issue Writer Certificate

Keywords

OXALIPLATINQ1

COLON CANCERQ1

CYTOTOXICITYQ1

APOPTOSISQ2

Abstract

Aim: The aim of this study is investigation of cell toxicity of OXALIPLATIN on COLON CANCER cell line and analysis of caspase 3 and 9 apoptotic genes.Material and methods: In this experimental study, CYTOTOXICITY of OXALIPLATIN on COLON CANCER cell line (HT29) was evaluated using MTT method in different concentrations including 100, 50, 25, 12.5, 6.25 and 3.125 μg/mL and the IC50 value was determined. After treatment of HT29 cells with IC50 value, the cells RNA was extracted and converted to cDNA. Subsequently, the expression level ofcaspase 3 and caspase 9 apoptotic genes comparing to house-keeping gene (b -actin) was measured using Real Time PCR.Results: Treatment of HT29 cells with various concentrations of OXALIPLATIN including show that OXALIPLATIN has the highest cytotoxic effect in 100 mg/mL, which is statistically significant (P<0.05). In addition to, the IC50 value of OXALIPLATIN was 6 mg/mL. Moreover, the expression ratio of caspase 3 and 9 genes comparing to b-actin gene in HT29 cells treated with OXALIPLATIN were up-regulated (2.69±0.72 (P<0.001), 3.26±0.56 (P<0.001), respectively).Conclusion: According to cell toxicity and APOPTOSIS induction in HT29 cells by OXALIPLATIN, it can be concluded that this drug is an appropriate choice for treating of COLON CANCER.

Cites

The Study of Expression of Casp3/Bax Genes in HT29 Colon Cancer Cell Line with Lactobacillus rhamnosus Co-Culturing

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APA: Copy

Yonesi, B., MIRZAIE, A., & ALIASGARI, E.. (2018). CYTOTOXICITY AND APOPTOTIC EFFECT OF OXALIPLATIN ON COLON CANCER CELL LINE (HT29) AND ANALYSIS OF CASPASE 3 AND CASPASE 9 GENE EXPRESSION USING REAL TIME PCR METHOD. JOURNAL OF CELL & TISSUE, 8(4 ), 364-373. SID. https://sid.ir/paper/189037/en

Vancouver: Copy

Yonesi B., MIRZAIE A., ALIASGARI E.. CYTOTOXICITY AND APOPTOTIC EFFECT OF OXALIPLATIN ON COLON CANCER CELL LINE (HT29) AND ANALYSIS OF CASPASE 3 AND CASPASE 9 GENE EXPRESSION USING REAL TIME PCR METHOD. JOURNAL OF CELL & TISSUE[Internet]. 2018;8(4 ):364-373. Available from: https://sid.ir/paper/189037/en

IEEE: Copy

B. Yonesi, A. MIRZAIE, and E. ALIASGARI, “CYTOTOXICITY AND APOPTOTIC EFFECT OF OXALIPLATIN ON COLON CANCER CELL LINE (HT29) AND ANALYSIS OF CASPASE 3 AND CASPASE 9 GENE EXPRESSION USING REAL TIME PCR METHOD,” JOURNAL OF CELL & TISSUE, vol. 8, no. 4 , pp. 364–373, 2018, [Online]. Available: https://sid.ir/paper/189037/en

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