THE CONCOMITANT EFFECT OF SHIKONIN AND GLUTATHIONE PEROXIDASE-1 ON ENHANCED SURVIVAL OF DOPAMINERGIC NEURONS AGAINST PARKINSONIAN TOXICITY

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ESMAEILZADEH E.; GARDANEH M.; VAZIRI H.R.

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Journal: JOURNAL OF CELL & TISSUE Year:2012 | Volume:3 | Issue:2 Page(s): 153-160

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Title

THE CONCOMITANT EFFECT OF SHIKONIN AND GLUTATHIONE PEROXIDASE-1 ON ENHANCED SURVIVAL OF DOPAMINERGIC NEURONS AGAINST PARKINSONIAN TOXICITY

Author(s)

ESMAEILZADEH E. | GARDANEH M. | VAZIRI H.R. | Issue Writer Certificate

Keywords

PAKINSONQ1

GLUTATHIONE PEROXIDASEQ1

SHIKONINQ2

DOPAMINERGIC NEURONQ2

Abstract

Aim: the aim of this study was to examine the effect of GLUTATHIONE PEROXIDASE-1 (GPX-1) and SHIKONIN on enhanced survival of DOPAMINERGIC NEURONs PC12 against parkinsonian toxicity.Material and methods: in order to overexpress GPX-1 in PC12 neurons, recombinant lentiviruses carrying both GPX-1 and reporter GFP genes were generated and used to infect target cells. The survival rate of the transduced neurons in the presence or absence of SHIKONIN was then quantified.Results: following GFP gene expression observed under the fluorescent microscope, the overexpression of GPX-1 was determined using the RT-PCR analysis. Changes in cell survival against parkinsonian toxicity were examined in the presence of two factors: GPX-1 overexpression and SHIKONIN treatment. The results indicated that both GPX-1 overexpression and SHIKONIN treatment of the PC12 cells increased significantly cell survival against parkinsonian toxicity. Survival increased by 14% after GPX-1 overexpression and by 11% following SHIKONIN treatment. More importantly, when the two factors were applied simultaneously (by SHIKONIN treatment of GPX-1-overexpressing cells) they saved 83% of the neuronal cells that was up by 29%. This increase of survival rate was significant compared to the increase achieved by each factor alone.Conclusion: our data showed that GPX-1 gene overexpression and SHIKONIN treatment not only individually increase PC12 cell survival against oxidative stress caused by the parkinonian toxin 6-OHDA, but also will function additively and/ or synergistically if they are applied together.

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APA: Copy

ESMAEILZADEH, E., GARDANEH, M., & VAZIRI, H.R.. (2012). THE CONCOMITANT EFFECT OF SHIKONIN AND GLUTATHIONE PEROXIDASE-1 ON ENHANCED SURVIVAL OF DOPAMINERGIC NEURONS AGAINST PARKINSONIAN TOXICITY. JOURNAL OF CELL & TISSUE, 3(2), 153-160. SID. https://sid.ir/paper/189064/en

Vancouver: Copy

ESMAEILZADEH E., GARDANEH M., VAZIRI H.R.. THE CONCOMITANT EFFECT OF SHIKONIN AND GLUTATHIONE PEROXIDASE-1 ON ENHANCED SURVIVAL OF DOPAMINERGIC NEURONS AGAINST PARKINSONIAN TOXICITY. JOURNAL OF CELL & TISSUE[Internet]. 2012;3(2):153-160. Available from: https://sid.ir/paper/189064/en

IEEE: Copy

E. ESMAEILZADEH, M. GARDANEH, and H.R. VAZIRI, “THE CONCOMITANT EFFECT OF SHIKONIN AND GLUTATHIONE PEROXIDASE-1 ON ENHANCED SURVIVAL OF DOPAMINERGIC NEURONS AGAINST PARKINSONIAN TOXICITY,” JOURNAL OF CELL & TISSUE, vol. 3, no. 2, pp. 153–160, 2012, [Online]. Available: https://sid.ir/paper/189064/en

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