INHIBITION OF LUNG CANCER CALU-6 CELL LINE PROLIFERATION USING CDC42 GENE SHRNA

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GHAMBARI Z.; NABIOUNI M.; JALALI H.; KARIMZADEH L.

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Journal: JOURNAL OF CELL & TISSUE Year:2017 | Volume:8 | Issue:3 Page(s): 261-270

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Title

INHIBITION OF LUNG CANCER CALU-6 CELL LINE PROLIFERATION USING CDC42 GENE SHRNA

Author(s)

GHAMBARI Z. | NABIOUNI M. | JALALI H. | KARIMZADEH L. | Issue Writer Certificate

Keywords

LENTIVIRUSQ2

LUNG CANCERQ2

GENE THERAPYQ1

SHRNAQ1

CDC42Q1

Abstract

Aim: In current study, we aimed to reduce CDC42 gene expression in lung carcinoma related cells, Calu-6, and assayed its effect on cell proliferation.Material and Methods: To reduce the expression of CDC42 gene SHRNA system was used and lentiviral system was selected to deliver CDC42 specific SHRNA to Calu-6 cells. Recombinant LENTIVIRUSes produced by co-transfection of pMD2G, psPAX2 and p-GFP-C-shLenti plasmids into 293T cells using lipofectamin. Efficiency of transfection and transduction assessed by florescent microscopy. Viability of cells treated by recombinant LENTIVIRUSes assessed by MTT assay.Results: florescent microscopy showed 80% transfection of 293T cells and high rate of Calu-6 cells transduction. MTT assay results revealed that viability of transduced Calu-6 cells reached to %58 and %40 in compare to control and negative control cells, respectively.Conclusion: recombinant LENTIVIRUSes properly transfer CDC42-shRNA into Calu-6 cells, leading to reduction of cell proliferation. Silencing of CDC42 gene expression using LENTIVIRUSes is persist and long-term effect which can be under attention for GENE THERAPY of LUNG CANCER.

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APA: Copy

GHAMBARI, Z., NABIOUNI, M., JALALI, H., & KARIMZADEH, L.. (2017). INHIBITION OF LUNG CANCER CALU-6 CELL LINE PROLIFERATION USING CDC42 GENE SHRNA. JOURNAL OF CELL & TISSUE, 8(3 ), 261-270. SID. https://sid.ir/paper/189088/en

Vancouver: Copy

GHAMBARI Z., NABIOUNI M., JALALI H., KARIMZADEH L.. INHIBITION OF LUNG CANCER CALU-6 CELL LINE PROLIFERATION USING CDC42 GENE SHRNA. JOURNAL OF CELL & TISSUE[Internet]. 2017;8(3 ):261-270. Available from: https://sid.ir/paper/189088/en

IEEE: Copy

Z. GHAMBARI, M. NABIOUNI, H. JALALI, and L. KARIMZADEH, “INHIBITION OF LUNG CANCER CALU-6 CELL LINE PROLIFERATION USING CDC42 GENE SHRNA,” JOURNAL OF CELL & TISSUE, vol. 8, no. 3 , pp. 261–270, 2017, [Online]. Available: https://sid.ir/paper/189088/en

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