LAMP DETECTION OF CRYPTOCOCCUS NEOFORMANS IN AIDS PATIENTS

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SHAH HOSSEINI MOHAMMAD HASAN; REZAEI AMIRABADI ALIREZA; VAND YOUSEFI JALIL; GHAHRI MOHAMMAD; AZADMANESH KEYHAN; MOSLEMI ELHAM

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Journal: JOURNAL OF MICROBIAL WORLD Year:2010 | Volume:2 | Issue:4 (5) Page(s): 235-242

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Title

LAMP DETECTION OF CRYPTOCOCCUS NEOFORMANS IN AIDS PATIENTS

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Keywords

LAMPQ3

CRYPTOCOCCUS NEOFORMANSQ3

AIDSQ2

Abstract

Background and Objective: CRYPTOCOCCUS NEOFORMANS is opportunistic capsulated yeast that infects immunocompromised individuals. Infection occurs due to inhalation of the agent and common infection form is meningitis. Several methods are common to detect this life threthening agent but none of them have well accepted in sensitivity, specificity and rapidness. In this research the effort has been on installation and optimizing the LAMP assay in order to detect the agent itself and its distribution importance of CRYPTOCOCCUS NEOFORMANS in serum specimens of AIDS patients.Material and methods: Atotal number of 34 serum specimens were collected from 34 AIDS patients. After applying sensitivity and specificity tests for the installed technique, optimization of the assay was performed on the specimens. The specimens got inactivated and after steps of DNA extraction, 6 designated primers for the target gene (ura5) were applyed for detection of CRYPTOCOCCUS NEOFORMANS by using the LAMP method.Results: In this study 3 specimens of the total number of 34 AIDS patients were reported positive by using LAMP technique. This it reveals the importance of Cryptococcal infection in AIDS patients.Conclution: Loop-mediated isothermal amplification is an applicable, sensitive, quick and lucrative method for detection of CRYPTOCOCCUS NEOFORMANS. Diagnostic laboratory can use this method without requiring any special instruments.

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APA: Copy

SHAH HOSSEINI, MOHAMMAD HASAN, REZAEI AMIRABADI, ALIREZA, VAND YOUSEFI, JALIL, GHAHRI, MOHAMMAD, AZADMANESH, KEYHAN, & MOSLEMI, ELHAM. (2010). LAMP DETECTION OF CRYPTOCOCCUS NEOFORMANS IN AIDS PATIENTS. JOURNAL OF MICROBIAL WORLD, 2(4 (5)), 235-242. SID. https://sid.ir/paper/189145/en

Vancouver: Copy

SHAH HOSSEINI MOHAMMAD HASAN, REZAEI AMIRABADI ALIREZA, VAND YOUSEFI JALIL, GHAHRI MOHAMMAD, AZADMANESH KEYHAN, MOSLEMI ELHAM. LAMP DETECTION OF CRYPTOCOCCUS NEOFORMANS IN AIDS PATIENTS. JOURNAL OF MICROBIAL WORLD[Internet]. 2010;2(4 (5)):235-242. Available from: https://sid.ir/paper/189145/en

IEEE: Copy

MOHAMMAD HASAN SHAH HOSSEINI, ALIREZA REZAEI AMIRABADI, JALIL VAND YOUSEFI, MOHAMMAD GHAHRI, KEYHAN AZADMANESH, and ELHAM MOSLEMI, “LAMP DETECTION OF CRYPTOCOCCUS NEOFORMANS IN AIDS PATIENTS,” JOURNAL OF MICROBIAL WORLD, vol. 2, no. 4 (5), pp. 235–242, 2010, [Online]. Available: https://sid.ir/paper/189145/en

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