GENERATION OF NEW GENE CONSTRUCTS FOR EXPRESSION OF OSTRICH GROWTH HORMONE GENE IN PROKARYOTIC AND EUKARYOTIC SYSTEMS

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FATHPOUR HOSSEIN; DOOSTI ABBAS; GHASEMI MARYAM; KABIRI HAMIDREZA

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Journal: JOURNAL OF MICROBIAL WORLD Year:2014 | Volume:7 | Issue:3 (20) Page(s): 190-196

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Title

GENERATION OF NEW GENE CONSTRUCTS FOR EXPRESSION OF OSTRICH GROWTH HORMONE GENE IN PROKARYOTIC AND EUKARYOTIC SYSTEMS

Author(s)

FATHPOUR HOSSEIN | DOOSTI ABBAS | GHASEMI MARYAM | KABIRI HAMIDREZA | Issue Writer Certificate

Keywords

OSTRICHQ1

GROWTH HORMONE (GH)Q1

CLONINGQ1

Abstract

Background & Objectives: Growth of OSTRICH depends to employment of growth hormone and proper nutrition. The OSTRICH growth hormone is a polypeptide that stimulates growth and cell reproduction. The aim of this study was to clone and generation of two new gene constructs for expression of OSTRICH growth hormone gene in prokaryotic and eukaryotic systems.Materials & Methods: In this study, RNA was extracted from OSTRICH pituitary gland and cDNAs synthesized using RT-PCR method. These cDNAs were cloned into TOPO vector based on T/A method. Then, cDNA sub-clones of OSTRICH growth hormone were prepared on pcDNA3.1 and pET32 vectors.Results: CLONING of OSTRICH growth hormone cDNA in TOPO vector was confirmed by PCR and endonuclease restriction digest. After sub-cloning of this cDNA, pcDNA3.1-gh and pET32-gh recombinant vectors were generated and the presence of genes were confirmed using restriction digestion.Conclusion: According to the results, the new recombinant vectors generated in this study can serve as an effective approach for production of the OSTRICH growth hormone in commercial levels.

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APA: Copy

FATHPOUR, HOSSEIN, DOOSTI, ABBAS, GHASEMI, MARYAM, & KABIRI, HAMIDREZA. (2014). GENERATION OF NEW GENE CONSTRUCTS FOR EXPRESSION OF OSTRICH GROWTH HORMONE GENE IN PROKARYOTIC AND EUKARYOTIC SYSTEMS. JOURNAL OF MICROBIAL WORLD, 7(3 (20)), 190-196. SID. https://sid.ir/paper/189167/en

Vancouver: Copy

FATHPOUR HOSSEIN, DOOSTI ABBAS, GHASEMI MARYAM, KABIRI HAMIDREZA. GENERATION OF NEW GENE CONSTRUCTS FOR EXPRESSION OF OSTRICH GROWTH HORMONE GENE IN PROKARYOTIC AND EUKARYOTIC SYSTEMS. JOURNAL OF MICROBIAL WORLD[Internet]. 2014;7(3 (20)):190-196. Available from: https://sid.ir/paper/189167/en

IEEE: Copy

HOSSEIN FATHPOUR, ABBAS DOOSTI, MARYAM GHASEMI, and HAMIDREZA KABIRI, “GENERATION OF NEW GENE CONSTRUCTS FOR EXPRESSION OF OSTRICH GROWTH HORMONE GENE IN PROKARYOTIC AND EUKARYOTIC SYSTEMS,” JOURNAL OF MICROBIAL WORLD, vol. 7, no. 3 (20), pp. 190–196, 2014, [Online]. Available: https://sid.ir/paper/189167/en

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