CLONING AND OVER EXPRESSION OF ALPHA-AMYLASE GENE ISOLATED FROM BACILLUS LICHENIFORMIS AND DETERMINATION OF BIOCHEMICAL CHARACTERIZATIONS OF THIS RECOMBINANT ENZYME

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KARIMINIK ASHRAF; SAEEDI KOBRA

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Journal: JOURNAL OF MICROBIAL WORLD Year:2014 | Volume:7 | Issue:3 (20) Page(s): 197-205

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Title

CLONING AND OVER EXPRESSION OF ALPHA-AMYLASE GENE ISOLATED FROM BACILLUS LICHENIFORMIS AND DETERMINATION OF BIOCHEMICAL CHARACTERIZATIONS OF THIS RECOMBINANT ENZYME

Author(s)

KARIMINIK ASHRAF | SAEEDI KOBRA | Issue Writer Certificate

Keywords

ALPHA AMYLASEQ2

PET-26B (+)Q2

BL21 (DE3)Q2

RECOMBINANT PROTEINQ2

Abstract

Background & Objectives: a-amylase, is an endoamylase to hydrolyses amylase, amylopectin and glycogen by randomly cleavage of internal alpha 1, 4 linkages. Genus Bacillus is one of the most common microbial sources of a-amylase production. The aim of the present study is identification, overexpression and activity analysis of Bacillus licheniformis a-amylase (amyS).Materials & Methods: A polymerase chain reaction was used to identify and isolate a-amylase gene in Bacillus sp. The fragments were introduced into expression vector pET-26 (+). After sequencing, the recombinant vectors were introduced into E. coli BL21 (DE3) for expression. The recombinant enzyme were purified using amicon filter and dialysis bags. a-amylase activity was measured using di-nitro salicylic acid technique.Results: Based on the results, the a-amylase gene was over-expressed in an expression system beyond the native host (Bacillus sp.). Based on the SDS-PAGE electrophoresis, the molecular weight of this enzyme was predicted 54 KDa. The a-amylase showed an enzyme activity about 4.77 U/ml.Conclusion: These results indicated a high expression of this enzyme in an expression system beyond the host, which was due to existence of a strong promoter used in this study, T7promoter. As a result, employment of this system in an industrial scale is recommended due to the secretion of the target enzyme, a proper folding of the enzyme and maintenance of its activity.

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APA: Copy

KARIMINIK, ASHRAF, & SAEEDI, KOBRA. (2014). CLONING AND OVER EXPRESSION OF ALPHA-AMYLASE GENE ISOLATED FROM BACILLUS LICHENIFORMIS AND DETERMINATION OF BIOCHEMICAL CHARACTERIZATIONS OF THIS RECOMBINANT ENZYME. JOURNAL OF MICROBIAL WORLD, 7(3 (20)), 197-205. SID. https://sid.ir/paper/189169/en

Vancouver: Copy

KARIMINIK ASHRAF, SAEEDI KOBRA. CLONING AND OVER EXPRESSION OF ALPHA-AMYLASE GENE ISOLATED FROM BACILLUS LICHENIFORMIS AND DETERMINATION OF BIOCHEMICAL CHARACTERIZATIONS OF THIS RECOMBINANT ENZYME. JOURNAL OF MICROBIAL WORLD[Internet]. 2014;7(3 (20)):197-205. Available from: https://sid.ir/paper/189169/en

IEEE: Copy

ASHRAF KARIMINIK, and KOBRA SAEEDI, “CLONING AND OVER EXPRESSION OF ALPHA-AMYLASE GENE ISOLATED FROM BACILLUS LICHENIFORMIS AND DETERMINATION OF BIOCHEMICAL CHARACTERIZATIONS OF THIS RECOMBINANT ENZYME,” JOURNAL OF MICROBIAL WORLD, vol. 7, no. 3 (20), pp. 197–205, 2014, [Online]. Available: https://sid.ir/paper/189169/en

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