OPTIMIZATION OF VARIABLE NUMBER TANDEM REPEAT (VNTR) ANALYSIS IN THE CLASSICAL PCR MACHINES FOR TYPING OF BURKHOLDERIA MALLEI

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NAJAFPOUR REZA; MOSAVARI NADER; TADAYON KEYVAN; TAJBAKHSH ELAHEH

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Journal: JOURNAL OF MICROBIAL WORLD Year:2015 | Volume:8 | Issue:3 (24) Page(s): 190-199

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Title

OPTIMIZATION OF VARIABLE NUMBER TANDEM REPEAT (VNTR) ANALYSIS IN THE CLASSICAL PCR MACHINES FOR TYPING OF BURKHOLDERIA MALLEI

Author(s)

NAJAFPOUR REZA | MOSAVARI NADER | TADAYON KEYVAN | TAJBAKHSH ELAHEH | Issue Writer Certificate

Keywords

BURKHOLDERIA MALLEIQ2

BM140Q2

BM1367Q2

BM2065Q2

BM2971Q2

Abstract

Background & Objectives: Glander is one of the important zoonotic diseases that is usually caused in solipeds including horse, donkey, mule, zebra and rarely in human. In the recent years, many molecular methods have been developed for the genome study of the BURKHOLDERIA MALLEI. The present study was aimed to optimize VNTR method on the basis of BM140, BM1367, BM2065 and BM2971 loci in order to molecular typing of B. mallei for the first time.Materials & Methods: In this study, B. mallei Razi 325, as a standard strain, was obtained from the microbial archive of the Razi Vaccine and Serum Research Institute. Following DNA extraction and PCR amplification of 4 loci, results of each locus was analysed independently and in different combinations. A special protocol was set in terms of temperature and concentrations of the cotenants (MgCl2 and primers) for each locus.Results: A comparison of the sequences of our isolate with the standard strains indicated the presence of polymorphism in these loci.Conclusion: Achievement of a common PCR protocol, which is able to proceed simultaneously four different reactions in one PCR run, was the primary outcome of this research.

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APA: Copy

NAJAFPOUR, REZA, MOSAVARI, NADER, TADAYON, KEYVAN, & TAJBAKHSH, ELAHEH. (2015). OPTIMIZATION OF VARIABLE NUMBER TANDEM REPEAT (VNTR) ANALYSIS IN THE CLASSICAL PCR MACHINES FOR TYPING OF BURKHOLDERIA MALLEI. JOURNAL OF MICROBIAL WORLD, 8(3 (24)), 190-199. SID. https://sid.ir/paper/189172/en

Vancouver: Copy

NAJAFPOUR REZA, MOSAVARI NADER, TADAYON KEYVAN, TAJBAKHSH ELAHEH. OPTIMIZATION OF VARIABLE NUMBER TANDEM REPEAT (VNTR) ANALYSIS IN THE CLASSICAL PCR MACHINES FOR TYPING OF BURKHOLDERIA MALLEI. JOURNAL OF MICROBIAL WORLD[Internet]. 2015;8(3 (24)):190-199. Available from: https://sid.ir/paper/189172/en

IEEE: Copy

REZA NAJAFPOUR, NADER MOSAVARI, KEYVAN TADAYON, and ELAHEH TAJBAKHSH, “OPTIMIZATION OF VARIABLE NUMBER TANDEM REPEAT (VNTR) ANALYSIS IN THE CLASSICAL PCR MACHINES FOR TYPING OF BURKHOLDERIA MALLEI,” JOURNAL OF MICROBIAL WORLD, vol. 8, no. 3 (24), pp. 190–199, 2015, [Online]. Available: https://sid.ir/paper/189172/en

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