MOLECULAR IDENTIFICATION OF ESCHERICHIA COLI K99 AND EVALUATION OF THE SERUM ANTIBODY TITER IN LABORATORY ANIMALS

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FARHANI FAEZEH; TAHAMTAN YAHYA; KARGAR MOHAMMAD

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Journal: JOURNAL OF MICROBIAL WORLD Year:2015 | Volume:8 | Issue:3 (24) Page(s): 200-208

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Title

MOLECULAR IDENTIFICATION OF ESCHERICHIA COLI K99 AND EVALUATION OF THE SERUM ANTIBODY TITER IN LABORATORY ANIMALS

Author(s)

FARHANI FAEZEH | TAHAMTAN YAHYA | KARGAR MOHAMMAD | Issue Writer Certificate

Keywords

FIMBRIAE K99Q2

ESCHERICHIA COLIQ1

DIARRHEAQ2

CALVESQ2

ELISAQ2

Abstract

Background & Objectives: ESCHERICHIA COLI is one of the primary etiologic agents for diarrhoea in neonatal CALVES, and the maternal immunization is an effective protection for the neonates because of feeding with colostrum. This study was aimed to molecular detection of E. coli K99 and evaluation of the serum antibody titers in laboratory animals.Materials & Methods: This study was experimentally carried out on rat to detect the marker genes. First, a Multiplex PCR was applied to detect the Sta, F41 and K99 pathogenic genes. Following growth of E. coli K99 bacteria into Minca broth, the bacteria were inactivated by 0.4% formaldehyde. The antigen was prepared after washing with PBS and centrifugation. The rats were subcutaneously inoculated with inactivated antigen with 107 and 109 CFU/ml in two groups. The control group was inoculated with a suspension containing all ingredients except for the antigen. Rat was allowed to nurse immediately after injection, and blood samples were taken for serum titration for 8 weeks.Results: An increase in the titer of serum antibody was seen after four weeks, and it continued after seven weeks. The antibody titration of rate was significantly higher in the immunized rat group than in the control group. We found good correlation in two groups. There is a grateful increase in antibody titer in group 2 (received 109 CFU/ml antigen) than in group 1 (received 107 CFU/ml antigen), but decreasing rate in the both groups were the same.Conclusion: These findings indicate that the use of killed antigen with high titer preparation containing sufficient antigen and may provide passive protection in animals. This resulted in by stimulation of immune cell due to high dose antigen.

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APA: Copy

FARHANI, FAEZEH, TAHAMTAN, YAHYA, & KARGAR, MOHAMMAD. (2015). MOLECULAR IDENTIFICATION OF ESCHERICHIA COLI K99 AND EVALUATION OF THE SERUM ANTIBODY TITER IN LABORATORY ANIMALS. JOURNAL OF MICROBIAL WORLD, 8(3 (24)), 200-208. SID. https://sid.ir/paper/189174/en

Vancouver: Copy

FARHANI FAEZEH, TAHAMTAN YAHYA, KARGAR MOHAMMAD. MOLECULAR IDENTIFICATION OF ESCHERICHIA COLI K99 AND EVALUATION OF THE SERUM ANTIBODY TITER IN LABORATORY ANIMALS. JOURNAL OF MICROBIAL WORLD[Internet]. 2015;8(3 (24)):200-208. Available from: https://sid.ir/paper/189174/en

IEEE: Copy

FAEZEH FARHANI, YAHYA TAHAMTAN, and MOHAMMAD KARGAR, “MOLECULAR IDENTIFICATION OF ESCHERICHIA COLI K99 AND EVALUATION OF THE SERUM ANTIBODY TITER IN LABORATORY ANIMALS,” JOURNAL OF MICROBIAL WORLD, vol. 8, no. 3 (24), pp. 200–208, 2015, [Online]. Available: https://sid.ir/paper/189174/en

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