Monitoring of biocement-and biogrout-producing bacteriain desert habitats of Iran

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KARGAR MEHDI; KARGAR MOHAMMAD

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Journal: JOURNAL OF MICROBIAL WORLD Year:2018 | Volume:11 | Issue:1 (34) Page(s): 51-60

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Information Journal Paper

Title

Monitoring of biocement-and biogrout-producing bacteriain desert habitats of Iran

Author(s)

KARGAR MEHDI | KARGAR MOHAMMAD | Issue Writer Certificate

Keywords

BiocementQ1

Sporosarcina pasteuriiQ1

UreaseQ2

16S rRNA geneQ1

Abstract

Background & Objectives: Microbially induced carbonate precipitation (MICP) is one of the proposed methods for soil stabilization and strengthening. The aim of this study was to isolate and characterize Biocement-producing ureolytic bacteria from arid soils. Materials & Methods: In this cross-sectional descriptive study, 280 soil samples were collected from different areas of Iran. Samples were cultured using urea broth medium. Urease-positive bacteria were assessed for the level of Urease activity. Identification of active strains was performed based on conventional biochemical tests and 16S rRNA gene sequencing. Optimum temperature and pH conditions for Biocement production were evaluated in Urease-positive bacteria and was compared with sporosaicina pasteurii (ATCC 11589) strain. Finally, calcium carbonate crystals were assessed byX-ray diffraction (XRD) crystallography test at the temperature of 25 ° C and pH of 8. 5 as optimal conditions. Results: 304 strains were isolated from 5 different bacterial genera. Bacillus and Sporosarcina were the major ureolytic isolates of arid regions. The most amount of calcium carbonate was produced at the temperature of 25 ° C and a pH of 8. 5. Ten most effective isolates in the production of Urease enzymes and Biocement were identified by molecular analysis and subsequently registered in NCBI Genbank. In addition, Biocement production by selected isolates was confirmed by XRD test. Conclusion: Our results showed that the amount of urea and calcium chloride plays an important role in calcium carbonate crystals production. S. pasteurii was one of the most important Biocement-producing bacteria. Therefore, optimization of growth conditions in higher scale is recommended for further applications in stabilizing the sand, ground strength and bridging.

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APA: Copy

KARGAR, MEHDI, & KARGAR, MOHAMMAD. (2018). Monitoring of biocement-and biogrout-producing bacteriain desert habitats of Iran. JOURNAL OF MICROBIAL WORLD, 11(1 (34) ), 51-60. SID. https://sid.ir/paper/189218/en

Vancouver: Copy

KARGAR MEHDI, KARGAR MOHAMMAD. Monitoring of biocement-and biogrout-producing bacteriain desert habitats of Iran. JOURNAL OF MICROBIAL WORLD[Internet]. 2018;11(1 (34) ):51-60. Available from: https://sid.ir/paper/189218/en

IEEE: Copy

MEHDI KARGAR, and MOHAMMAD KARGAR, “Monitoring of biocement-and biogrout-producing bacteriain desert habitats of Iran,” JOURNAL OF MICROBIAL WORLD, vol. 11, no. 1 (34) , pp. 51–60, 2018, [Online]. Available: https://sid.ir/paper/189218/en

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