ISOLATION AND SEQUENCING OF MERCURY RESISTANCE GENE IN RAOULTELLA PLANTICULA BACTERIA

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REZAEI ASHRAF; MOGHBELI MAJID

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Journal: JOURNAL OF MICROBIAL WORLD Year:2017 | Volume:9 | Issue:4 (29) Page(s): 307-314

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Title

ISOLATION AND SEQUENCING OF MERCURY RESISTANCE GENE IN RAOULTELLA PLANTICULA BACTERIA

Author(s)

REZAEI ASHRAF | MOGHBELI MAJID | Issue Writer Certificate

Keywords

MERA GENEQ1

MERCURYQ2

PCRQ2

RAOULTELLA PLANTICULA.Q1

Abstract

Background & Objectives: MERCURY is one of the polluting sources of the environment, plants as well as human. Microorganisms are resistant to MERCURY through different mechanisms. The main mechanism involves reduction of the toxic MERCURY to the elemental form which is mediated through a variety of bacterial genes. The aim of this study is to investigate the presence of MERCURY resistance gene in RAOULTELLA PLANTICULA.bacteria using polymerase chain reaction (PCR) and sequencing methods.Materials & Methods: Genomic and plasmid DNA was extracted from wastewater R. planticula isolates. PCR was set up with primers designed based on the presence of mer A gene in Klebsiella oxytoca. The PCR products were sequenced and analyzed using NCBI database.Results: Using PCR on plasmid and genomic bacterial DNA, a 1700 bps bond was obtained. Blasting the sequence, it was found that the amplified gene has 99% sequence homology to MERA GENE in Enterobacter cloacae, E. coli, Serratia marcescens, Klebsiella oxytoca and Acinetobacter.Conclusion: For the first time in this study, we found that RAOULTELLA PLANTICULA.isolated from Iran natural sources are resistant to MERCURY due to the presence of MERA GENE. Therefore, R. planticula can be considered an appropriate candidate to reduce or remove MERCURY from industrial wastewaters.

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APA: Copy

REZAEI, ASHRAF, & MOGHBELI, MAJID. (2017). ISOLATION AND SEQUENCING OF MERCURY RESISTANCE GENE IN RAOULTELLA PLANTICULA BACTERIA. JOURNAL OF MICROBIAL WORLD, 9(4 (29)), 307-314. SID. https://sid.ir/paper/189225/en

Vancouver: Copy

REZAEI ASHRAF, MOGHBELI MAJID. ISOLATION AND SEQUENCING OF MERCURY RESISTANCE GENE IN RAOULTELLA PLANTICULA BACTERIA. JOURNAL OF MICROBIAL WORLD[Internet]. 2017;9(4 (29)):307-314. Available from: https://sid.ir/paper/189225/en

IEEE: Copy

ASHRAF REZAEI, and MAJID MOGHBELI, “ISOLATION AND SEQUENCING OF MERCURY RESISTANCE GENE IN RAOULTELLA PLANTICULA BACTERIA,” JOURNAL OF MICROBIAL WORLD, vol. 9, no. 4 (29), pp. 307–314, 2017, [Online]. Available: https://sid.ir/paper/189225/en

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