GENOTYPING OF STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM NOSO-COMIAL INFECTIONS BY MULTILOCUS SEQUENCE TYPING (MLST)

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RAFEE SARA; TAJBAKHSH ELAHEH; MOMTAZ HASSAN

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Journal: JOURNAL OF MICROBIAL WORLD Year:2017 | Volume:9 | Issue:4 (29) Page(s): 278-285

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Title

GENOTYPING OF STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM NOSO-COMIAL INFECTIONS BY MULTILOCUS SEQUENCE TYPING (MLST)

Author(s)

RAFEE SARA | TAJBAKHSH ELAHEH | MOMTAZ HASSAN | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS EPIDERMIDISQ2

HOUSEKEEPING GENESQ1

GENETIC CLONESQ1

MULTILOCUS SEQUENCE TYPING (MLST)Q1

Abstract

Background & Objectives: STAPHYLOCOCCUS EPIDERMIDISis one of the main causes of urinary tract infections and second cause of respiratory infections in human. The aim of this study was genotyping S. epidermidis strains isolated from nosocomial infections and detection of GENETIC CLONES using Multilocus Sequence Typing (MLST) method.Materials & Methods: In this cross-sectional study, 16 S. epidermidis isolates were selected and PCR products from amplification of seven HOUSEKEEPING GENES were sequenced. The nucleotide sequences of each gene in each isolate were analyzed in the MLST database and, besides identifying different clones, gene - specific alleles in each sequence types (ST) were determined.Results: A total of 3 clones including ST22, ST88 and ST153 were identified from 16 isolates, which was classified into two gene clusters of A and B. ST22 clone with a frequency of 50%, ST88 with 31.25% and ST153 with 18.75% were identified. The most dominant S. epidermidis clone isolated in 16 isolates is ST22.Conclusion: Dendrogram analysis of the isolates showed the homology of all isolates to alleles previously reported. Furthermore, our results suggest the genetic diversity of the isolates.

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APA: Copy

RAFEE, SARA, TAJBAKHSH, ELAHEH, & MOMTAZ, HASSAN. (2017). GENOTYPING OF STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM NOSO-COMIAL INFECTIONS BY MULTILOCUS SEQUENCE TYPING (MLST). JOURNAL OF MICROBIAL WORLD, 9(4 (29)), 278-285. SID. https://sid.ir/paper/189231/en

Vancouver: Copy

RAFEE SARA, TAJBAKHSH ELAHEH, MOMTAZ HASSAN. GENOTYPING OF STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM NOSO-COMIAL INFECTIONS BY MULTILOCUS SEQUENCE TYPING (MLST). JOURNAL OF MICROBIAL WORLD[Internet]. 2017;9(4 (29)):278-285. Available from: https://sid.ir/paper/189231/en

IEEE: Copy

SARA RAFEE, ELAHEH TAJBAKHSH, and HASSAN MOMTAZ, “GENOTYPING OF STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM NOSO-COMIAL INFECTIONS BY MULTILOCUS SEQUENCE TYPING (MLST),” JOURNAL OF MICROBIAL WORLD, vol. 9, no. 4 (29), pp. 278–285, 2017, [Online]. Available: https://sid.ir/paper/189231/en

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