EVALUATION OF XYLITOL SWEETENER PRODUCTION BY CANDIDA GUILLIERMONDII

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ASGARY KARCHEGANY FOROUGH; GHEZELBASH GHOLAM REZA; NAHVI IRAJ

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Journal: JOURNAL OF MICROBIAL WORLD Year:2015 | Volume:7 | Issue:4 (21) Page(s): 329-338

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Title

EVALUATION OF XYLITOL SWEETENER PRODUCTION BY CANDIDA GUILLIERMONDII

Author(s)

ASGARY KARCHEGANY FOROUGH | GHEZELBASH GHOLAM REZA | NAHVI IRAJ | Issue Writer Certificate

Keywords

XYLITOLQ2

XYLOSEQ2

CANDIDA GUILLIERMONDIIQ2

Abstract

Background & Objectives: XYLITOL is a naturally five–carbon polyol with a high sweetening ability. The biotechnological production of XYLITOL by microorganisms specially yeasts is very environmentally safe and has been studied as an alternative to the chemical method. The purpose of this study was XYLITOL production by CANDIDA GUILLIERMONDII that isolated from spores of Zinnia.Materials & Methods: The XYLITOL producing yeast strain was isolated on Yeast extract Pepton XYLOSE (YePX) medium. The identity of strains was confirmed using PCR reaction. The XYLITOL produced by the strains was analyzed using tine layer chromatography (TLC) and specific kits. The concentration of XYLITOL produced in this experiment was quantified using HPLC method.Results: The XYLITOL producing yeast strain was isolated from pollen of Zinnia elegans flower and was identified by 16S rRNA sequence analysis as CANDIDA GUILLIERMONDII. This strain produced 24.67 g l-1 XYLITOL after 72 hours in medium contained 40 g l-1 XYLOSE.Conclusion: In this study XYLITOL was produced by CANDIDA GUILLIERMONDII isolated from the nature. According to the properties of XYLITOL and the difficulties through chemical production of this compound, the sweetener produced by biotechnological methods can be used as an attractive sugar in the nutritional supplement and pharmaceutical industries.

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APA: Copy

ASGARY KARCHEGANY, FOROUGH, GHEZELBASH, GHOLAM REZA, & NAHVI, IRAJ. (2015). EVALUATION OF XYLITOL SWEETENER PRODUCTION BY CANDIDA GUILLIERMONDII. JOURNAL OF MICROBIAL WORLD, 7(4 (21)), 329-338. SID. https://sid.ir/paper/189244/en

Vancouver: Copy

ASGARY KARCHEGANY FOROUGH, GHEZELBASH GHOLAM REZA, NAHVI IRAJ. EVALUATION OF XYLITOL SWEETENER PRODUCTION BY CANDIDA GUILLIERMONDII. JOURNAL OF MICROBIAL WORLD[Internet]. 2015;7(4 (21)):329-338. Available from: https://sid.ir/paper/189244/en

IEEE: Copy

FOROUGH ASGARY KARCHEGANY, GHOLAM REZA GHEZELBASH, and IRAJ NAHVI, “EVALUATION OF XYLITOL SWEETENER PRODUCTION BY CANDIDA GUILLIERMONDII,” JOURNAL OF MICROBIAL WORLD, vol. 7, no. 4 (21), pp. 329–338, 2015, [Online]. Available: https://sid.ir/paper/189244/en

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