OPTIMIZATION OF REAL-TIME PCR METHOD TO ASSESS THE DIRECT SENSITIVITY OF CLARITHROMYCIN IN HELICOBACTER PYLORI

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GHORBANI DALINI SADEGH; KARGAR MOHAMMAD; DOOSTI ABBAS; ABBASI PEJMAN; SOUOD NEGAR

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Journal: JOURNAL OF MICROBIAL WORLD Year:2009 | Volume:2 | Issue:3 Page(s): 149-154

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Title

OPTIMIZATION OF REAL-TIME PCR METHOD TO ASSESS THE DIRECT SENSITIVITY OF CLARITHROMYCIN IN HELICOBACTER PYLORI

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Keywords

HELICOBACTER PYLORIQ2

CLARITHROMYCINQ3

REAL-TIME PCRQ2

Abstract

Background and Objectives: The increasing rate of CLARITHROMYCIN resistance in H. pylori is recognized as a significant contributing factor for treatment failure. The aim of this study was to representing a REAL-TIME PCR assay in comparison with disk diffusion method for detection of CLARITHROMYCIN resistance in H. pylori.Materials and Methods: This Experimental study was performed on 45 H. pylori specimens isolated from gastrointestinal disorders patients. In all samples, presence of H. pylori was confirmed by using culture, rapid urease test and conventional PCR. Also, CLARITHROMYCIN resistance were assessed by using CLSI (Clinical and Laboratory Standard Institute) protocol and REAL-TIME PCR using specific primers and probe for H. pylori peptidylteransferas region of 23S rRNA gene.Results: Disk diffusion method identified 20 (44.44%) samples as susceptible and 25 (55.56%) samples as resistance to CLARITHROMYCIN. But, real time PCR were identified samples as 20 (44.44%) with susceptible genotype, 5 (11.11%) with mix genotype and 20 (44.44%) as resistance to CLARITHROMYCIN genotype. Also results showed that sensitivity of this assay was equal to 40 bacteria or 80 copy number of 23S rRNA gene.Conclusion: Results showed that REAL-TIME PCR assay has high accuracy for identifying CLARITHROMYCIN resistance in short time.

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APA: Copy

GHORBANI DALINI, SADEGH, KARGAR, MOHAMMAD, DOOSTI, ABBAS, ABBASI, PEJMAN, & SOUOD, NEGAR. (2009). OPTIMIZATION OF REAL-TIME PCR METHOD TO ASSESS THE DIRECT SENSITIVITY OF CLARITHROMYCIN IN HELICOBACTER PYLORI. JOURNAL OF MICROBIAL WORLD, 2(3), 149-154. SID. https://sid.ir/paper/189291/en

Vancouver: Copy

GHORBANI DALINI SADEGH, KARGAR MOHAMMAD, DOOSTI ABBAS, ABBASI PEJMAN, SOUOD NEGAR. OPTIMIZATION OF REAL-TIME PCR METHOD TO ASSESS THE DIRECT SENSITIVITY OF CLARITHROMYCIN IN HELICOBACTER PYLORI. JOURNAL OF MICROBIAL WORLD[Internet]. 2009;2(3):149-154. Available from: https://sid.ir/paper/189291/en

IEEE: Copy

SADEGH GHORBANI DALINI, MOHAMMAD KARGAR, ABBAS DOOSTI, PEJMAN ABBASI, and NEGAR SOUOD, “OPTIMIZATION OF REAL-TIME PCR METHOD TO ASSESS THE DIRECT SENSITIVITY OF CLARITHROMYCIN IN HELICOBACTER PYLORI,” JOURNAL OF MICROBIAL WORLD, vol. 2, no. 3, pp. 149–154, 2009, [Online]. Available: https://sid.ir/paper/189291/en

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