RAPID AND SIMULTANEOUS IDENTITY OF VIRULENCE FACTORS AND CAPSULAR TYPING OF PASTEURELLA MULTOCIDA ISOLATED FROM SHEEP AND GOATS BY MULTIPLEX PCR

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DANESH LARI SAMANEH; TAHAMTAN YAHYA; HAYATI MASOUME; KARGAR MOHAMMAD

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Journal: JOURNAL OF MICROBIAL WORLD Year:2010 | Volume:3 | Issue:3 (8) Page(s): 162-168

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Title

RAPID AND SIMULTANEOUS IDENTITY OF VIRULENCE FACTORS AND CAPSULAR TYPING OF PASTEURELLA MULTOCIDA ISOLATED FROM SHEEP AND GOATS BY MULTIPLEX PCR

Author(s)

DANESH LARI SAMANEH | TAHAMTAN YAHYA | HAYATI MASOUME | KARGAR MOHAMMAD | Issue Writer Certificate

Keywords

PASTEURELLA MULTOCIDAQ3

VIRULENCE FACTORQ3

MULTIPLEX PCRQ1

SHEEPQ2

GOATSQ2

Abstract

Background and objective: PASTEURELLA MULTOCIDA exist commensally in respiratory track of a variety of wild and domestic animals. Serotypes A and D are responsible for pneumonia in GOATS and SHEEP, and Fimbriae, adhesions, capsule, toxin, iron uptake factor and outer membrane protein are its important VIRULENCE FACTORs. The aim of this study was to design a MULTIPLEX PCR for fast and simultaneous detection of more important VIRULENCE FACTORs of P. multocida isolated from SHEEP and GOATS as well as their capsular typing and identification.Materials and Methods: Totally 500 nasal swabs were collected from the GOATS and SHEEPs suspected to respiratory signs of pasteurellasis. After isolation abd detection of P. multosida by biochemical examination, the bacteria were assayed by specific primers for identification of major important VIRULENCE FACTORs and their capsular typing.Result: Capsular typing of the isolates by PCR showed two capsular types A, D with 83.8% and 6.4% prevalence, respectively. The results also showed that 23 (74.19%), 21 (67.74%), 21 (67.74%) and 24 (77.4%) of the isolates were positive for presence of toxA, hgbA, ptfA and ompH genes, respectively.Conclusion: The most isolated P. multocida from goat and SHEEP were toxigenic, and capsular type A was the most common isolates in the Fars province. The remarkable high prevalence of toxA and ompH among the afflicted SHEEP and GOATS may imply to important role of these genes in epidemiology and virulence of P. multocida. Furthermore the high prevalence of P. multocida typeA harbor toxA gene can be attributed to its important role in the respiratory infection.

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APA: Copy

DANESH LARI, SAMANEH, TAHAMTAN, YAHYA, HAYATI, MASOUME, & KARGAR, MOHAMMAD. (2010). RAPID AND SIMULTANEOUS IDENTITY OF VIRULENCE FACTORS AND CAPSULAR TYPING OF PASTEURELLA MULTOCIDA ISOLATED FROM SHEEP AND GOATS BY MULTIPLEX PCR. JOURNAL OF MICROBIAL WORLD, 3(3 (8)), 162-168. SID. https://sid.ir/paper/189324/en

Vancouver: Copy

DANESH LARI SAMANEH, TAHAMTAN YAHYA, HAYATI MASOUME, KARGAR MOHAMMAD. RAPID AND SIMULTANEOUS IDENTITY OF VIRULENCE FACTORS AND CAPSULAR TYPING OF PASTEURELLA MULTOCIDA ISOLATED FROM SHEEP AND GOATS BY MULTIPLEX PCR. JOURNAL OF MICROBIAL WORLD[Internet]. 2010;3(3 (8)):162-168. Available from: https://sid.ir/paper/189324/en

IEEE: Copy

SAMANEH DANESH LARI, YAHYA TAHAMTAN, MASOUME HAYATI, and MOHAMMAD KARGAR, “RAPID AND SIMULTANEOUS IDENTITY OF VIRULENCE FACTORS AND CAPSULAR TYPING OF PASTEURELLA MULTOCIDA ISOLATED FROM SHEEP AND GOATS BY MULTIPLEX PCR,” JOURNAL OF MICROBIAL WORLD, vol. 3, no. 3 (8), pp. 162–168, 2010, [Online]. Available: https://sid.ir/paper/189324/en

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