ISOLATION AND CLONING OF THE CUTINASE GENE OBTAINED FROM INDIGENOUS THERMOPHILES THERMOBIFIDA FUSCA BACTERIA

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POURFAKHRAEI ELAHE; BEHMANESH MEHRDAD; ERFANI MOGHADAM MAJID

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Journal: JOURNAL OF MICROBIAL WORLD Year:2010 | Volume:3 | Issue:3 (8) Page(s): 155-161

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Title

ISOLATION AND CLONING OF THE CUTINASE GENE OBTAINED FROM INDIGENOUS THERMOPHILES THERMOBIFIDA FUSCA BACTERIA

Author(s)

POURFAKHRAEI ELAHE | BEHMANESH MEHRDAD | ERFANI MOGHADAM MAJID | Issue Writer Certificate

Keywords

THERMOBIFIDA FUSCAQ3

CUTINASEQ3

CLONINGQ2

Abstract

Background and Objectives: CUTINASE belongs to serine hydrolase family that is used for elimination of environmental pollutions due to its special structural and functional properties and applications. In this study, CUTINASE gene was isolated from indigenous thermophiles THERMOBIFIDA FUSCA and was cloned in E. coli.Material and Methods: Sampling was performed from forest soils in the north of Iran. THERMOBIFIDA FUSCA bacteria were isolated with suitable cultures under optimized conditions (pH 10 and T=62oC). After primer designation, cut1 gene was isolated from bacterium and then, was cloned in pBluescript sk vector and was introduced into E. coli DH5a. The colonization was confirmed based on enzymatic digestion and sequencing.Result: Based on BLAST software, there was 98% and 96% similarities were obtained with cut1 and cut-1.KW3, respectively.Conclusions: Based on broad functions of the enzyme on various substrates and its ability for synthesis of various polyester compounds, this enzyme has an important role in environmental decontamination.

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APA: Copy

POURFAKHRAEI, ELAHE, BEHMANESH, MEHRDAD, & ERFANI MOGHADAM, MAJID. (2010). ISOLATION AND CLONING OF THE CUTINASE GENE OBTAINED FROM INDIGENOUS THERMOPHILES THERMOBIFIDA FUSCA BACTERIA. JOURNAL OF MICROBIAL WORLD, 3(3 (8)), 155-161. SID. https://sid.ir/paper/189326/en

Vancouver: Copy

POURFAKHRAEI ELAHE, BEHMANESH MEHRDAD, ERFANI MOGHADAM MAJID. ISOLATION AND CLONING OF THE CUTINASE GENE OBTAINED FROM INDIGENOUS THERMOPHILES THERMOBIFIDA FUSCA BACTERIA. JOURNAL OF MICROBIAL WORLD[Internet]. 2010;3(3 (8)):155-161. Available from: https://sid.ir/paper/189326/en

IEEE: Copy

ELAHE POURFAKHRAEI, MEHRDAD BEHMANESH, and MAJID ERFANI MOGHADAM, “ISOLATION AND CLONING OF THE CUTINASE GENE OBTAINED FROM INDIGENOUS THERMOPHILES THERMOBIFIDA FUSCA BACTERIA,” JOURNAL OF MICROBIAL WORLD, vol. 3, no. 3 (8), pp. 155–161, 2010, [Online]. Available: https://sid.ir/paper/189326/en

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