EVALUATION OF ADHERENCE OF PROBIOTIC LACTOBACILUS CASEITO HEP2- CELL LINE (SHORT COMMUNICATION)

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TAKALLOO ZEINAB; GOUDARZVAND MAHDI; KHODAII ZOHREH

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Journal: JOURNAL OF MICROBIAL WORLD Year:2016 | Volume:9 | Issue:1 (26) Page(s): 71-75

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Title

EVALUATION OF ADHERENCE OF PROBIOTIC LACTOBACILUS CASEITO HEP2- CELL LINE (SHORT COMMUNICATION)

Author(s)

TAKALLOO ZEINAB | GOUDARZVAND MAHDI | KHODAII ZOHREH | Issue Writer Certificate

Keywords

PROBIOTICSQ2

ADHESIONQ1

HEP-2 CELLSQ1

LACTOBACILLUS CASEIQ2

Abstract

The first step to choose a strain as probiotic is capability for the bacteria to adhere to mucosal ADHESION due to increase the prevention of the adherence of pathogenic bacteria, and the secretion of antimicrobial agents, which produce an appropriate condition of microbial growth. This study was aimed to evaluate the adherence ability of Lactobacilus casei to Hep-2 cell line. First, the HEP-2 CELLS were grown in RPMI medium containing fetal calf serum and antibiotics. The defined numbers of probiotic bacteria grown in MRS broth were added to the HEp-2 cell. Then, the cells were washed after attachment of bacteria to the plates. Thereafter, the cells were stained and fixed with histological methods. The ADHESION ability of bacteria and the pattern of ADHESION was observed under optical microscope. Our results showed that L. casei was able to adhere to oro- pharyngeal cell line. Also, the optimum time to test the ability was 3 hours. These results indicated that the probiotic bacterium L. casei is suitable for application in health products. Based on these results, we recommend application of L. casei in oral hygiene products.

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APA: Copy

TAKALLOO, ZEINAB, GOUDARZVAND, MAHDI, & KHODAII, ZOHREH. (2016). EVALUATION OF ADHERENCE OF PROBIOTIC LACTOBACILUS CASEITO HEP2- CELL LINE (SHORT COMMUNICATION). JOURNAL OF MICROBIAL WORLD, 9(1 (26)), 71-75. SID. https://sid.ir/paper/189343/en

Vancouver: Copy

TAKALLOO ZEINAB, GOUDARZVAND MAHDI, KHODAII ZOHREH. EVALUATION OF ADHERENCE OF PROBIOTIC LACTOBACILUS CASEITO HEP2- CELL LINE (SHORT COMMUNICATION). JOURNAL OF MICROBIAL WORLD[Internet]. 2016;9(1 (26)):71-75. Available from: https://sid.ir/paper/189343/en

IEEE: Copy

ZEINAB TAKALLOO, MAHDI GOUDARZVAND, and ZOHREH KHODAII, “EVALUATION OF ADHERENCE OF PROBIOTIC LACTOBACILUS CASEITO HEP2- CELL LINE (SHORT COMMUNICATION),” JOURNAL OF MICROBIAL WORLD, vol. 9, no. 1 (26), pp. 71–75, 2016, [Online]. Available: https://sid.ir/paper/189343/en

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