MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY

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KHODAYARI SOMAYEH; KAFILZADEH FARSHID

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Journal: JOURNAL OF MICROBIAL WORLD Year:2017 | Volume:10 | Issue:3 (32) Page(s): 231-242

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Title

MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY

Author(s)

KHODAYARI SOMAYEH | KAFILZADEH FARSHID | Issue Writer Certificate

Keywords

KERATINASE ENZYMEQ1

FEATHER-DEGRADING BACTERIAQ1

BACILLUS CEREUSQ2

Abstract

Background & Objectives: Feather is one of the environmental pollutants that about 90% of its weight is made of highly resistant creatine plates. A number of bacteria are able to produce a KERATINASE ENZYME in the presence of keratin substrate. The purpose of this study was isolation and molecular identification of keratin- degrading bacteria from the soil of poultry farms around Marvdasht city in order to measure keratinase activity in superior creatine-degrading strains.Material & Methods: In this study, 15 soil samples from poultry farms around Marvdasht city was collected. Seven bacterial isolates were cultured on a feather meal medium. Five isolates that showed clear degradation zone, were chosen for further chemical and molecular identification. The bacteria were sequenced, and a specific accession number in the gene bank was allocated to each of them as a new strain Then, all five isolates were assessed for the level of KERATINASE ENZYME production.Result: All isolates belonged to Bacillus species. All five isolates were able to completely degrade keratine. The most keratinase activity was reported 17.12 (ml/min) for BACILLUS CEREUS SKH1.Conclusion: Different species of Bacillus in this study showed the ability to produce a KERATINASE ENZYME in the presence of keratin substrate. Investigating the level of keratinase activity in bacterial isolates showed that all have the potential treat feather waste.

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APA: Copy

KHODAYARI, SOMAYEH, & KAFILZADEH, FARSHID. (2017). MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY. JOURNAL OF MICROBIAL WORLD, 10(3 (32) ), 231-242. SID. https://sid.ir/paper/189345/en

Vancouver: Copy

KHODAYARI SOMAYEH, KAFILZADEH FARSHID. MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY. JOURNAL OF MICROBIAL WORLD[Internet]. 2017;10(3 (32) ):231-242. Available from: https://sid.ir/paper/189345/en

IEEE: Copy

SOMAYEH KHODAYARI, and FARSHID KAFILZADEH, “MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY,” JOURNAL OF MICROBIAL WORLD, vol. 10, no. 3 (32) , pp. 231–242, 2017, [Online]. Available: https://sid.ir/paper/189345/en

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