Identification of resistant eggplant cultivars to Fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt using molecular markers in Iran

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Safikhani Negin; MORID BAHAR; ZAMANIZADEH HAMIDREZA; HAJMANSOOR SHAHAB

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Journal: JOURNAL OF MICROBIAL WORLD Year:2019 | Volume:11 | Issue:4 (37) Page(s): 392-403

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Title

Identification of resistant eggplant cultivars to Fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt using molecular markers in Iran

Author(s)

Safikhani Negin | MORID BAHAR | ZAMANIZADEH HAMIDREZA | HAJMANSOOR SHAHAB | Issue Writer Certificate

Keywords

Fusarium wiltQ2

EggplantQ3

Molecular markersQ2

Resistance geneQ2

Abstract

Background & Objectives: Eggplant Fusarium wiltis an important factor of yield reduction throughout the world. The ability of this pathogen to survive for several consecutive years within the soil, even in the absence of the host, has made it difficult to control. Producing and using the resistant cultivar is the most effective and suitable method to control this disease. This study was aimed to identify Fusarium wilt resistant Eggplant cultivars. Materials & Methods: First, leaf samples of domestic and hybrid Eggplant cultivars were gathered from 28 provinces in Iran and then DNA extraction from young leaves of the cultivars was carried out using CTAB method. Four markers including CAPS, RAPD, SRAP, and SCAR were used to determine the resistant cultivars. In order to confirm the results, resistance and sensitivity of the genotypes were assessed in greenhouse conditions, as well. Results: Out of 20 genotypes of this study, 13 showed index resistance band using CAPS, RAPD, and SRAP of Molecular markers. On the other hand, the SCAR marker could not separate the resistant cultivars from the sensitive ones. Phenotype assessment of native and hybrids resistant cultivars in greenhouse condition confirmed the results of the molecular analysis. Conclusion: In general, the use of resistant cultivars obtained in this study using Molecular markers is recommended for planting in areas with Fusarium wilt disease.

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APA: Copy

Safikhani, Negin, MORID, BAHAR, ZAMANIZADEH, HAMIDREZA, & HAJMANSOOR, SHAHAB. (2019). Identification of resistant eggplant cultivars to Fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt using molecular markers in Iran. JOURNAL OF MICROBIAL WORLD, 11(4 (37) ), 392-403. SID. https://sid.ir/paper/189358/en

Vancouver: Copy

Safikhani Negin, MORID BAHAR, ZAMANIZADEH HAMIDREZA, HAJMANSOOR SHAHAB. Identification of resistant eggplant cultivars to Fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt using molecular markers in Iran. JOURNAL OF MICROBIAL WORLD[Internet]. 2019;11(4 (37) ):392-403. Available from: https://sid.ir/paper/189358/en

IEEE: Copy

Negin Safikhani, BAHAR MORID, HAMIDREZA ZAMANIZADEH, and SHAHAB HAJMANSOOR, “Identification of resistant eggplant cultivars to Fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt using molecular markers in Iran,” JOURNAL OF MICROBIAL WORLD, vol. 11, no. 4 (37) , pp. 392–403, 2019, [Online]. Available: https://sid.ir/paper/189358/en

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