ISOLATION AND MOLECULAR IDENTIFICATION OF L-ASPARAGINASE PRODUCING BACTERIA FROM PERSIAN GULF

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IZADPANAH QESHMI FATEMEH; JAVADPOUR SEDIGHEH; MALEKZADEH KIANOOSH

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Journal: JOURNAL OF MICROBIAL WORLD Year:2013 | Volume:6 | Issue:3 (16) Page(s): 237-245

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Title

ISOLATION AND MOLECULAR IDENTIFICATION OF L-ASPARAGINASE PRODUCING BACTERIA FROM PERSIAN GULF

Author(s)

IZADPANAH QESHMI FATEMEH | JAVADPOUR SEDIGHEH | MALEKZADEH KIANOOSH | Issue Writer Certificate

Keywords

L-ASPARAGINASEQ2

16S RRNAQ2

PERSIAN GULFQ1

Abstract

Background and Objectives: L-ASPARAGINASE is an anti-neoblastic agent used in the chemotherapy of acute lymphoblastic leukemia: This enzyme is widely distributed among microorganisms and animals. Microorganisms have proved to be a better alternative for L-ASPARAGINASE. The objective of this study was isolation and molecular identification of L-ASPARAGINASE producing bacteria from PERSIAN GULF.Material and Methods: In this study, samples were collected both water and sediments of PERSIAN GULF. An screening culture was performed on M9 in order to isolate the L- asparaginase producing bacteria. Next, after preparation of bacterial cell free suspension, the L-ASPARAGINASE activity was measured by Colorimertic method. Among producing L-ASPARAGINASE strains 13 isolates with high L-ASPARAGINASE activity were selected and their phylogeny were detected based on nucleotide sequence of 16S RRNA gene.Results: Totally, 57 isolates out of 181 achieved bacterial colonies were able to produce L-ASPARAGINASE. Based on 16SrRNA analysis, these 13 chosen isolates with high L-ASPARAGINASE producing ability belonged to Bacillus spp. (8 isolate, 61.5%), Pseudomonas spp. (2 isolate, 15.4%), Zobellella spp. (1 isolate, 7.7%), Oceanimonas spp. (1 isolate, 7.7%) and Acinetobacter (1 isolate, 7.7%). The highest and lowest L-ASPARAGINASE productivity was measured in Pseudomonas sp. PG-Ol with 1.6 IU and Acinetobacter sp. PG-14 with 0.07 ID, respectively.Conclusion: Some marine bacterial strains isolated from PERSIAN GULF are potential source of L-ASPARAGINASE enzyme. Pseudomonas sp. PG-01is especially useful for commercial production of this enzyme. In this study, the ability of Zobellella spp. for production of L asparaginase is reported for first time.

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APA: Copy

IZADPANAH QESHMI, FATEMEH, JAVADPOUR, SEDIGHEH, & MALEKZADEH, KIANOOSH. (2013). ISOLATION AND MOLECULAR IDENTIFICATION OF L-ASPARAGINASE PRODUCING BACTERIA FROM PERSIAN GULF. JOURNAL OF MICROBIAL WORLD, 6(3 (16)), 237-245. SID. https://sid.ir/paper/189395/en

Vancouver: Copy

IZADPANAH QESHMI FATEMEH, JAVADPOUR SEDIGHEH, MALEKZADEH KIANOOSH. ISOLATION AND MOLECULAR IDENTIFICATION OF L-ASPARAGINASE PRODUCING BACTERIA FROM PERSIAN GULF. JOURNAL OF MICROBIAL WORLD[Internet]. 2013;6(3 (16)):237-245. Available from: https://sid.ir/paper/189395/en

IEEE: Copy

FATEMEH IZADPANAH QESHMI, SEDIGHEH JAVADPOUR, and KIANOOSH MALEKZADEH, “ISOLATION AND MOLECULAR IDENTIFICATION OF L-ASPARAGINASE PRODUCING BACTERIA FROM PERSIAN GULF,” JOURNAL OF MICROBIAL WORLD, vol. 6, no. 3 (16), pp. 237–245, 2013, [Online]. Available: https://sid.ir/paper/189395/en

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