PRODUCTION AND PARTIAL PURIFICATION OF STREPTAVIDIN (SA) FROM BACTERIAL SUPERNATANT BY DEAE-CELLULOSE IONEXCHANGE CHROMATOGRAPHY

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JAMIE R.; MIRSHAHI M.; NADERIMANESH H.

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Journal: Year:2004 | Volume:17 | Issue:1 (62 IN ANIMAL AND FISHERIES SCIENCES) Page(s): 21-26

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Title

PRODUCTION AND PARTIAL PURIFICATION OF STREPTAVIDIN (SA) FROM BACTERIAL SUPERNATANT BY DEAE-CELLULOSE IONEXCHANGE CHROMATOGRAPHY

Author(s)

JAMIE R. | MIRSHAHI M. | NADERIMANESH H. | Issue Writer Certificate

Keywords

STREPTAVIDINQ4

STREPTOMYCES AVIDINIQ4

PURIFICATIONQ3

DEAE-CELLULOSEQ4

Abstract

Streptavid in (SA) is a protein secreted by STREPTOMYCES AVIDINI. It has four subunits and each subunit has high affinity for biotin. In the present research, we have analysed the effect of the defined (A&B) and undefined (LB & LB+Asn) culture media onthe growth of S.avidini and the amount of protein secreted SA in bacterial supematant was concentrated with ammonium sulfate and then partially purified using a DEAE-CELLULOSE column. The purity of SA was evaluated by SDS-PAGE and immunoblotting. Our results showed that the best culture conditions was in LB medium at 32°C for ten days where the yield of SA was about 13mg/l and the purified SA could be used directly.

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APA: Copy

JAMIE, R., MIRSHAHI, M., & NADERIMANESH, H.. (2004). PRODUCTION AND PARTIAL PURIFICATION OF STREPTAVIDIN (SA) FROM BACTERIAL SUPERNATANT BY DEAE-CELLULOSE IONEXCHANGE CHROMATOGRAPHY. PAJOUHESH-VA-SAZANDEGI, 17(1 (62 IN ANIMAL AND FISHERIES SCIENCES)), 21-26. SID. https://sid.ir/paper/19280/en

Vancouver: Copy

JAMIE R., MIRSHAHI M., NADERIMANESH H.. PRODUCTION AND PARTIAL PURIFICATION OF STREPTAVIDIN (SA) FROM BACTERIAL SUPERNATANT BY DEAE-CELLULOSE IONEXCHANGE CHROMATOGRAPHY. PAJOUHESH-VA-SAZANDEGI[Internet]. 2004;17(1 (62 IN ANIMAL AND FISHERIES SCIENCES)):21-26. Available from: https://sid.ir/paper/19280/en

IEEE: Copy

R. JAMIE, M. MIRSHAHI, and H. NADERIMANESH, “PRODUCTION AND PARTIAL PURIFICATION OF STREPTAVIDIN (SA) FROM BACTERIAL SUPERNATANT BY DEAE-CELLULOSE IONEXCHANGE CHROMATOGRAPHY,” PAJOUHESH-VA-SAZANDEGI, vol. 17, no. 1 (62 IN ANIMAL AND FISHERIES SCIENCES), pp. 21–26, 2004, [Online]. Available: https://sid.ir/paper/19280/en

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