DIAGNOSIS OF CRYPTOCOCCAL MENINGITIDIS BY STAINING, CULTURE AND PCR

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SHAHHOSSEINY M.H.; GHAHRI M.; NASROLLAHI SHIRAZI SH.; SAADATMAND S.; HOSSEINI S.A.

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Journal: JOURNAL OF MICROBIOLOGY KNOWLEDGE Year:2010 | Volume:2 | Issue:5 Page(s): 39-46

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Title

DIAGNOSIS OF CRYPTOCOCCAL MENINGITIDIS BY STAINING, CULTURE AND PCR

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Keywords

CRYPTOCOCCUS NEOFORMANSQ4

POLYMERASE CHAIN REACTIONQ4

CEREBRO SPINAL FLUIDQ4

MENINGITISQ4

Abstract

CRYPTOCOCCUS NEOFORMANS is encapsulated opportunistic yeast which usually affects the individuals with immune deficiency but can also cause disease in healthy individuals with a worldwide distribution. Infection emerges following inhalation of fungi and MENINGITIS is the common clinical form of the disease. There are several common methods for the diagnosis of this factor, but none of these methods have acceptable sensitivity, specificity and speed. Molecular methods like PCR have appropriate sensitivity, specificity and speed that can be relied on as a suitable method in the diagnosis of CRYPTOCOCCUS NEOFORMANS. Cerebrospinal fluid samples of 140 suspicious patients to meningitidis which were collected from a total of 5 hospitals were cultivated, stained and PCR was performed. A sensitive PCR using primers ITS1 and CN4 and 16S rRNA as target gene was designed and used on cerebrospinal fluid samples collected from patients. Culture and staining (Indian ink) of all samples were negative. In one sample (related to HIV-positive patient who was suffering from MENINGITIS), PCR result was positive. Current molecular detection limit was assessed as 10 organisms per ml CRYPTOCOCCUS NEOFORMANS. Specificity test was performed with different species of Candida and Aspergillus, as well as common bacteria causing MENINGITIS and no product was proliferated. This study indicates that PCR is a sensitive and reliable method for rapid diagnosis of CRYPTOCOCCUS NEOFORMANS in cerebrospinal fluid samples.

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APA: Copy

SHAHHOSSEINY, M.H., GHAHRI, M., NASROLLAHI SHIRAZI, SH., SAADATMAND, S., & HOSSEINI, S.A.. (2010). DIAGNOSIS OF CRYPTOCOCCAL MENINGITIDIS BY STAINING, CULTURE AND PCR. JOURNAL OF MICROBIOLOGY KNOWLEDGE, 2(5), 39-46. SID. https://sid.ir/paper/193201/en

Vancouver: Copy

SHAHHOSSEINY M.H., GHAHRI M., NASROLLAHI SHIRAZI SH., SAADATMAND S., HOSSEINI S.A.. DIAGNOSIS OF CRYPTOCOCCAL MENINGITIDIS BY STAINING, CULTURE AND PCR. JOURNAL OF MICROBIOLOGY KNOWLEDGE[Internet]. 2010;2(5):39-46. Available from: https://sid.ir/paper/193201/en

IEEE: Copy

M.H. SHAHHOSSEINY, M. GHAHRI, SH. NASROLLAHI SHIRAZI, S. SAADATMAND, and S.A. HOSSEINI, “DIAGNOSIS OF CRYPTOCOCCAL MENINGITIDIS BY STAINING, CULTURE AND PCR,” JOURNAL OF MICROBIOLOGY KNOWLEDGE, vol. 2, no. 5, pp. 39–46, 2010, [Online]. Available: https://sid.ir/paper/193201/en

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