Efficient production and application of egg yolk antibody powder against Staphylococcus aureus

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Aliakbari Farima; MAHDAVI AMIR HOSSEIN; RAHMANI HAMID REZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Animal Science Year:2018 | Volume:49 | Issue:1 Page(s): 63-71

Download Full-Text

Persian Verion

View:

551

Download:

Cites:

Information Journal Paper

Title

Efficient production and application of egg yolk antibody powder against Staphylococcus aureus

Author(s)

Aliakbari Farima | MAHDAVI AMIR HOSSEIN | RAHMANI HAMID REZA | Issue Writer Certificate

Keywords

Antibacterial effectsQ1

immunoglobulin YQ1

IgY extractionQ1

Abstract

The aim of present experiment was to investigate the immunochemical characteristics as well as in vitro inhibitory effects of specific and nonspecific egg yolk antibody powder on Staphylococcus aureus growth, as one of the main pathogenic serotypes of Staphylococcus in human and animals. A total of 16 White Leghorn hens (Hy-Line W36) of forty weeks in age were immunized by killed sonicated vaccine after plotting the growth curve and incubating the bacteria until the end of exponential growth phase. This vaccine was prepared eggs were collected daily for 8 weeks, IgY was then isolated and lyophilized by Polson method. The immunochemical properties and growth inhibitory effects of specific and nonspecific sIgY were tested against Staphylococcus aureus. The results showed that the plasma and egg specific activity of IgY against Staphylococcus aureus markedly increased after the first immunization and remained on the highest titer throughout the experimental period (P<0. 01). Total IgY concentration and purity of specific IgY powder were 71% higher rather than the non-specific IgY powder (P<0. 05). The administration of specific IgY powder at 100 and 200 mg significantly (P<0. 05) reduced the bacterial counts during the incubation hours of 4 and 6. Compared to control group, using the highest level of specific IgY could significantly decrease 1. 20 log CFU/mL (P<0. 01). Therefore, the present findings indicated that Staphylococcus aureus accompanying with Freund’ s adjuvant (complete form in the first immunization and incomplete form in booster immunization) have a notable potential to produce high antibody levels for a long time period. In addition, Polson method is an effective way to obtain remarkable purity for IgY with high antibacterial efficiency and long term production.

Cites

No record.

References

No record.

Cite

APA: Copy

Aliakbari, Farima, MAHDAVI, AMIR HOSSEIN, & RAHMANI, HAMID REZA. (2018). Efficient production and application of egg yolk antibody powder against Staphylococcus aureus. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(1 ), 63-71. SID. https://sid.ir/paper/193521/en

Vancouver: Copy

Aliakbari Farima, MAHDAVI AMIR HOSSEIN, RAHMANI HAMID REZA. Efficient production and application of egg yolk antibody powder against Staphylococcus aureus. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2018;49(1 ):63-71. Available from: https://sid.ir/paper/193521/en

IEEE: Copy

Farima Aliakbari, AMIR HOSSEIN MAHDAVI, and HAMID REZA RAHMANI, “Efficient production and application of egg yolk antibody powder against Staphylococcus aureus,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 1 , pp. 63–71, 2018, [Online]. Available: https://sid.ir/paper/193521/en

Related Journal Papers