PRODUCTION OF CHICKEN EGG YOLK IMMUNOGLOBULIN AGAINST HELICOBACTER PYLORI UREC PROTEIN BY DNA VACCINATION

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AMIRI JAVID SH.; MOUSAVI GARGARI S.L.; SALMANIAN ALI HATEF; BASIRI MOHSEN

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Journal: KOWSAR MEDICAL JOURNAL Year:2011 | Volume:15 | Issue:4 Page(s): 197-202

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Title

PRODUCTION OF CHICKEN EGG YOLK IMMUNOGLOBULIN AGAINST HELICOBACTER PYLORI UREC PROTEIN BY DNA VACCINATION

Author(s)

AMIRI JAVID SH. | MOUSAVI GARGARI S.L. | SALMANIAN ALI HATEF | BASIRI MOHSEN | Issue Writer Certificate

Keywords

HELICOBACTER PYLORIQ3

EGG YOLK IMMUNOGLOBULIN (IGY)Q3

UREASEQ3

DNA VACCINEQ3

Abstract

Aims: Current therapies against HELICOBACTER PYLORI infection, which is associated with gastritis, peptic ulcer and gastric cancer, are performed by antibiotic use. Due to increase of antibiotic resistance and difficulty and cost of treatment, new approaches have focused on using specific antibodies. Accordingly, efficiency of antibodies specific to H. pylori UREASE has been demonstrated. Considering the longtime benefit and efficacy of DNA vaccination, this study was designed on order to use DNA vaccination for generation of egg yolk immunoglobulin against UreC subunit of H. pylori UREASE.Materials & Methods: H. pylori ureC gene was cloned into eukaryotic pCI expression vector. Recombinant pCI-ureC plasmid, amplified in E. coli host were purified and used for genetic immunization of chickens. IgY recovered from egg yolk, using Poly Ethylene Glycol precipitation and analyzed by indirect ELISA and UREASE test.Results: Using DNA vaccination, specific and biologically active IgY antibodies were produced and extracted, which based on ELISA results is able to detect UreC subunit of UREASE.Conclusion: DNA immunization can be used as a productive and economic method to generate efficient antiurease antibodies for therapeutic proposes.

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APA: Copy

AMIRI JAVID, SH., MOUSAVI GARGARI, S.L., SALMANIAN, ALI HATEF, & BASIRI, MOHSEN. (2011). PRODUCTION OF CHICKEN EGG YOLK IMMUNOGLOBULIN AGAINST HELICOBACTER PYLORI UREC PROTEIN BY DNA VACCINATION. KOWSAR MEDICAL JOURNAL, 15(4), 197-202. SID. https://sid.ir/paper/32872/en

Vancouver: Copy

AMIRI JAVID SH., MOUSAVI GARGARI S.L., SALMANIAN ALI HATEF, BASIRI MOHSEN. PRODUCTION OF CHICKEN EGG YOLK IMMUNOGLOBULIN AGAINST HELICOBACTER PYLORI UREC PROTEIN BY DNA VACCINATION. KOWSAR MEDICAL JOURNAL[Internet]. 2011;15(4):197-202. Available from: https://sid.ir/paper/32872/en

IEEE: Copy

SH. AMIRI JAVID, S.L. MOUSAVI GARGARI, ALI HATEF SALMANIAN, and MOHSEN BASIRI, “PRODUCTION OF CHICKEN EGG YOLK IMMUNOGLOBULIN AGAINST HELICOBACTER PYLORI UREC PROTEIN BY DNA VACCINATION,” KOWSAR MEDICAL JOURNAL, vol. 15, no. 4, pp. 197–202, 2011, [Online]. Available: https://sid.ir/paper/32872/en

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