EFFECT OF CARBOHYDRATE, GIBBERELLIC ACID, INDOLEBUTYRIC ACID, PHLOROGLUCINOL, EXPLANT ORIENTATION AND CULTURE VESSELS VOLUME ON OPTIMIZING IN VITRO PROPAGATION OF M.9 APPLE ROOTSTOCK

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MIRI S.M.; VAEZ LIVARI B.; KHALIGHI A.; GHAEM MAGHAMI S.A.

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Journal Paper

Paper Information

Journal: Year:2003 | Volume:16 | Issue:2 (59 IN AGRONOMY AND HORTICULTURE) Page(s): 31-37

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Information Journal Paper

Title

EFFECT OF CARBOHYDRATE, GIBBERELLIC ACID, INDOLEBUTYRIC ACID, PHLOROGLUCINOL, EXPLANT ORIENTATION AND CULTURE VESSELS VOLUME ON OPTIMIZING IN VITRO PROPAGATION OF M.9 APPLE ROOTSTOCK

Author(s)

MIRI S.M. | VAEZ LIVARI B. | KHALIGHI A. | GHAEM MAGHAMI S.A. | Issue Writer Certificate

Keywords

APPLEQ2

PROLIFERATIONQ2

CARBOHYDRATEQ2

GIBBERELLIC ACIDQ2

INDOLE-3-BUTYRIC ACIDQ2

PHLOROGLUCINOLQ3

EXPLANT QRIENTATIONQ4

CULTURE VESSELQ4

Abstract

In this research, with the purpose to optimize in vitro shoot PROLIFERATION of .9, the factors of CARBOHYDRATE, GIBBERELLIC ACID (GA3), ndolebutyric acid (IBA), PHLOROGLUCINOL (PG), explant orientation and CULTURE VESSELs volume were studied. The basal medium was MS supplemented with 1.5 mg.l-1 BA and 1 mg.l -1 K in. Shoot explants 1 cm in length was taken from in vitro samples. The results showed that sorbitol was more suitable as a CARBOHYDRATE for multiplication than sucrose. GA3 and PG had no effect on number of shoots, but affected length of shoots. Application of IBA had no effect on PROLIFERATION. Explants orientation has significant effect on PROLIFERATION and the best results were obtained with vertical orientation. Increase in vessel volume from 25 ml to 125 ml had no effect on shoots PROLIFERATION of M.9. On the basis of the results, is suggested for improving of M.9 PROLIFERATION were used from basal medium culture (MS with 1.5 mg.l -1 BA, 1mg.l-1 Kin and 8 g.l -1 agar) containing 30 g.l -1 sorbitol, 0.1 mg.l -1 GA3, 162 mg.l-1 PG. Also, shoots put in medium vertically.

Cites

EFFECT OF MEDIUM TYPE AND SOME BIOCHEMICAL COMPONENTS ON IN VITRO PROLIFERATION OF DWARF ROOTSTOCK OF APPLE (MALUS DOMESTICA BORKH CV GAMI ALMASI)

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APA: Copy

MIRI, S.M., VAEZ LIVARI, B., KHALIGHI, A., & GHAEM MAGHAMI, S.A.. (2003). EFFECT OF CARBOHYDRATE, GIBBERELLIC ACID, INDOLEBUTYRIC ACID, PHLOROGLUCINOL, EXPLANT ORIENTATION AND CULTURE VESSELS VOLUME ON OPTIMIZING IN VITRO PROPAGATION OF M.9 APPLE ROOTSTOCK. PAJOUHESH-VA-SAZANDEGI, 16(2 (59 IN AGRONOMY AND HORTICULTURE)), 31-37. SID. https://sid.ir/paper/19468/en

Vancouver: Copy

MIRI S.M., VAEZ LIVARI B., KHALIGHI A., GHAEM MAGHAMI S.A.. EFFECT OF CARBOHYDRATE, GIBBERELLIC ACID, INDOLEBUTYRIC ACID, PHLOROGLUCINOL, EXPLANT ORIENTATION AND CULTURE VESSELS VOLUME ON OPTIMIZING IN VITRO PROPAGATION OF M.9 APPLE ROOTSTOCK. PAJOUHESH-VA-SAZANDEGI[Internet]. 2003;16(2 (59 IN AGRONOMY AND HORTICULTURE)):31-37. Available from: https://sid.ir/paper/19468/en

IEEE: Copy

S.M. MIRI, B. VAEZ LIVARI, A. KHALIGHI, and S.A. GHAEM MAGHAMI, “EFFECT OF CARBOHYDRATE, GIBBERELLIC ACID, INDOLEBUTYRIC ACID, PHLOROGLUCINOL, EXPLANT ORIENTATION AND CULTURE VESSELS VOLUME ON OPTIMIZING IN VITRO PROPAGATION OF M.9 APPLE ROOTSTOCK,” PAJOUHESH-VA-SAZANDEGI, vol. 16, no. 2 (59 IN AGRONOMY AND HORTICULTURE), pp. 31–37, 2003, [Online]. Available: https://sid.ir/paper/19468/en

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