STUDY OF THE PHYSIOLOGICAL EFFECTS OF NITROGEN FIXATION BY AGROBACTERIUM TUMEFACIENES IN THREE MEDICAGO CULTIVARS

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KERAMAT B.; MANOUCHEHR KALANTARI KH.; NAMAKI SHOUSHTARI A.A.H.

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Journal: Year:2003 | Volume:15 | Issue:3-4 (56-57 IN AGRONOMY AND HORTICULTURE) Page(s): 88-90

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Title

STUDY OF THE PHYSIOLOGICAL EFFECTS OF NITROGEN FIXATION BY AGROBACTERIUM TUMEFACIENES IN THREE MEDICAGO CULTIVARS

Author(s)

KERAMAT B. | MANOUCHEHR KALANTARI KH. | NAMAKI SHOUSHTARI A.A.H. | Issue Writer Certificate

Keywords

AGROBACTERIUMQ2

NITROGEN FIXATIONQ3

MEDICAGO SATIVAQ3

Abstract

Many studies had been done on the microorganisms which were able to fix nitrogen recently. AGROBACTERIUM has been added to this list. AGROBACTERIUM belong to the family Rhizobiaceae. For study of the role of this bacterium in nirtogen fixation, the effects of this system on three different cultivars of Medicago namely Bami, Australian and American were investigated. In this study either mutate Sinorhizobium meliloti, which lack plasmid (nifH-nod+, nifK-) or carries plasmid nifH-nod+(pSRk9) was used and compared with wild type and control. Analysis of variance showed that there was not any significant differences in height of those plants inoculated with nifH- when compared with control plant, but a significant increase in length was observed in plants inoculated with nifK-(PSRK9).The results showed that when plants were inoculated with sinorhizobium which contain nirtogen fixing system nifK-(PSRK9), the net production increased in all cultivars (based on dry weight). Sinorhizobium which carries plasmid, especially nifK-(SPRK9) had much higher number of active and large nodules which indicates nifK- is effective in NITROGEN FIXATION. For determination of the quality of proteins which resulted from direct effects of NITROGEN FIXATION in AGROBACTERIUM electrophoresis system was used the results indicated that there is not any differences in protein type. Sinorhzobium which carries plasmid of that lack plasmid but significant differences in total protein which accumulated in plants inoculated with different sinorhizobium was observed.

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APA: Copy

KERAMAT, B., MANOUCHEHR KALANTARI, KH., & NAMAKI SHOUSHTARI, A.A.H.. (2003). STUDY OF THE PHYSIOLOGICAL EFFECTS OF NITROGEN FIXATION BY AGROBACTERIUM TUMEFACIENES IN THREE MEDICAGO CULTIVARS. PAJOUHESH-VA-SAZANDEGI, 15(3-4 (56-57 IN AGRONOMY AND HORTICULTURE)), 88-90. SID. https://sid.ir/paper/19493/en

Vancouver: Copy

KERAMAT B., MANOUCHEHR KALANTARI KH., NAMAKI SHOUSHTARI A.A.H.. STUDY OF THE PHYSIOLOGICAL EFFECTS OF NITROGEN FIXATION BY AGROBACTERIUM TUMEFACIENES IN THREE MEDICAGO CULTIVARS. PAJOUHESH-VA-SAZANDEGI[Internet]. 2003;15(3-4 (56-57 IN AGRONOMY AND HORTICULTURE)):88-90. Available from: https://sid.ir/paper/19493/en

IEEE: Copy

B. KERAMAT, KH. MANOUCHEHR KALANTARI, and A.A.H. NAMAKI SHOUSHTARI, “STUDY OF THE PHYSIOLOGICAL EFFECTS OF NITROGEN FIXATION BY AGROBACTERIUM TUMEFACIENES IN THREE MEDICAGO CULTIVARS,” PAJOUHESH-VA-SAZANDEGI, vol. 15, no. 3-4 (56-57 IN AGRONOMY AND HORTICULTURE), pp. 88–90, 2003, [Online]. Available: https://sid.ir/paper/19493/en

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