THE EFFECT OF PHYTOHEMAGGLUTININ (PHA) AND CYTOKININES INTERACTIONS ON THE CALLUS PRODUCTION AND ORGANOGENESIS OF NICOTIANA PLANT TISSUE

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JAVADI HAMIDEH

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Journal: Year:2003 | Volume:15 | Issue:3-4 (56-57 IN AGRONOMY AND HORTICULTURE) Page(s): 62-70

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Title

THE EFFECT OF PHYTOHEMAGGLUTININ (PHA) AND CYTOKININES INTERACTIONS ON THE CALLUS PRODUCTION AND ORGANOGENESIS OF NICOTIANA PLANT TISSUE

Author(s)

JAVADI HAMIDEH | Issue Writer Certificate

Keywords

CYTOKININQ2

PHYTOHEMAGGLUTININQ4

ORGANOGENESISQ4

TISSUE CULTUREQ3

Abstract

In this research the interaction of PHYTOHEMAGGLUTININ (PHA) with CYTOKININes Banzil-Adenine, Forfiril- min and Adenine on the callus production and ORGANOGENESIS of Nicotiana plant tissue was investigated. For this purpose MS basal medium and different concentrations of CYTOKININes and PHA were applied. Two levels (2 and 3 ppm) of PHA and two levels (0.1 and 1 ppm) of CYTOKININes were used. The experiment was conducted as a completely randomized design with three replication. Explants from fifth leaf lamina of Nicotiana tabacum were selected and cultured on the culture medium. Subsequently, explants transferred to 26±2 degree of centigrade under 16 h. daylight with 1500 lux intensity. The fresh and dry weight explants after fourty days were recorded. Because of heteroscedasticity within treatments in the experiment, the treatments were divided into two groups (groups one and two) and analysis of variance was performed for each group separately. Statistical analysis showed significant differences among the treatments in the two groups for fresh weight, but for dry weight the differences were observed among the first group. Comparison of treatment means by Duncan's - multiple range test (p=%5) indicated that the treatment of (BA 1ppmx PAH 2ppm) and treatment of (BA 1ppmxPHA 3ppm) for fresh weight and treatment of (FA 1ppmxPHA 3ppm), (BA 1ppmxPHA 2ppm), (BA 1ppmxPHA 3ppm) and (Fd 1ppmxPHA 2ppm) for dry weight were superior than other treatments. So the treatments with BA 1ppm or FA 1ppm and 2 or 3ppm of PHA showed fresh and dry weight more than other treatments, and produce seedling from steam and root.

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APA: Copy

JAVADI, HAMIDEH. (2003). THE EFFECT OF PHYTOHEMAGGLUTININ (PHA) AND CYTOKININES INTERACTIONS ON THE CALLUS PRODUCTION AND ORGANOGENESIS OF NICOTIANA PLANT TISSUE. PAJOUHESH-VA-SAZANDEGI, 15(3-4 (56-57 IN AGRONOMY AND HORTICULTURE)), 62-70. SID. https://sid.ir/paper/19496/en

Vancouver: Copy

JAVADI HAMIDEH. THE EFFECT OF PHYTOHEMAGGLUTININ (PHA) AND CYTOKININES INTERACTIONS ON THE CALLUS PRODUCTION AND ORGANOGENESIS OF NICOTIANA PLANT TISSUE. PAJOUHESH-VA-SAZANDEGI[Internet]. 2003;15(3-4 (56-57 IN AGRONOMY AND HORTICULTURE)):62-70. Available from: https://sid.ir/paper/19496/en

IEEE: Copy

HAMIDEH JAVADI, “THE EFFECT OF PHYTOHEMAGGLUTININ (PHA) AND CYTOKININES INTERACTIONS ON THE CALLUS PRODUCTION AND ORGANOGENESIS OF NICOTIANA PLANT TISSUE,” PAJOUHESH-VA-SAZANDEGI, vol. 15, no. 3-4 (56-57 IN AGRONOMY AND HORTICULTURE), pp. 62–70, 2003, [Online]. Available: https://sid.ir/paper/19496/en

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