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Information Journal Paper

Title

IDENTITY TESTING WITH FOURTEEN GENETIC MARKERS OF ARABIAN HORSES IN CHARMAHAL-VA-BAKHTIARI PROVINCE

Pages

  49-56

Abstract

 Nowadays with develop molecular genetic techniques, beings identification and parentage testing is possible using a lot of molecular markers. STR are simple sequences of DNA consisting of short tandem repeats have a high polymorphism, and for this reason, they are used as powerful tools for recognizing the identity. These sequences the total 20% of constitute in mammifera. The aim of this study is examine the fourteen GENETIC MARKERS AHT5, AHT4 ASB23, ASB17, ASB2, VHL20, CA425, HMS7, HMS6, HMS3, HMS1, HTG4, HTG10 and LEX3 of Arabian horses  and use they in IDENTITY TESTING it species in Chaharmahal-va-bakhtiari province. Blood samples collected from 13 families Arabian strain in Chaharmahal-va-bakhtiari province area and DNA was extracted the Multiplex polymerase chain reaction was used for amplification of fourteen markers with the specific primers and the PCR products were resolved on a non-denaturing 10% polyacrylamide gel by electrophoresis, then the PCR products remaining obtainable with formamide and electrophoresis was carried out on an ABI PRISM 3100 Genetic Analyzer using the recommended protocols. DNA fragments separated were performed with genotype software Ver.3.7. The number of alleles per locus varied from 3 to 9 (average 6.36) in arabian horse. The Polymorphic information contents average 6.41 and the homozygosity was average 0.683 In population total. Since that those markers in all of the studying horse strain, have various kinds of DNA fragments. Consequently it seems that these foueteen markers can be used as an applicable marker for identifying Arabian horse.

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    APA: Copy

    DOOSTI, ABBAS, JAVAHERI KOUPAEI, MOHAMMAD, & MOSHKELANI, SAADAT. (2010). IDENTITY TESTING WITH FOURTEEN GENETIC MARKERS OF ARABIAN HORSES IN CHARMAHAL-VA-BAKHTIARI PROVINCE. JOURNAL OF VETERINARY PATHOBIOLOGY, 1(2), 49-56. SID. https://sid.ir/paper/195524/en

    Vancouver: Copy

    DOOSTI ABBAS, JAVAHERI KOUPAEI MOHAMMAD, MOSHKELANI SAADAT. IDENTITY TESTING WITH FOURTEEN GENETIC MARKERS OF ARABIAN HORSES IN CHARMAHAL-VA-BAKHTIARI PROVINCE. JOURNAL OF VETERINARY PATHOBIOLOGY[Internet]. 2010;1(2):49-56. Available from: https://sid.ir/paper/195524/en

    IEEE: Copy

    ABBAS DOOSTI, MOHAMMAD JAVAHERI KOUPAEI, and SAADAT MOSHKELANI, “IDENTITY TESTING WITH FOURTEEN GENETIC MARKERS OF ARABIAN HORSES IN CHARMAHAL-VA-BAKHTIARI PROVINCE,” JOURNAL OF VETERINARY PATHOBIOLOGY, vol. 1, no. 2, pp. 49–56, 2010, [Online]. Available: https://sid.ir/paper/195524/en

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