DISTRIBUTION OF STAPHYLOCOCCAL ENTEROTOXIN A GENE AMONG STAPHYLOCOCCUS AUREUS ISOLATES FROM TRADITIONAL WHITE-BRINED CHEESE

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MOHAMMADI KH.

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Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2015 | Volume:11 (NEW) | Issue:4 (47) Page(s): 1473-1479

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Title

DISTRIBUTION OF STAPHYLOCOCCAL ENTEROTOXIN A GENE AMONG STAPHYLOCOCCUS AUREUS ISOLATES FROM TRADITIONAL WHITE-BRINED CHEESE

Author(s)

MOHAMMADI KH. | Issue Writer Certificate

Keywords

TRADITIONAL WHITE–BRINED CHEESEQ2

STAPHYLOCOCCUS AUREUSQ1

GENEQ1

ENTEROTOXINQ1

MULTIPLEX PCRQ2

Abstract

The consumption of food containing staphylococcal ENTEROTOXINs is regularly identified as the cause of intoxication. ENTEROTOXIN A is considered as the most common toxin in staphylococcus–related food poisoning. The purpose of this study was to determine the prevalence of S. aureus in traditional white-brined cheese and distribution of ENTEROTOXIN A GENE (sea) among them. A total of 120 samples was examined and S. aureus was isolated from 11 (9.1%) of the samples. According to the results, load of S. aureus was estimated from 1.5×101 to 8.6×104 cfu/g. No sample was in the critical cell density of >105 cfu/g. From each sample, five suspected colonies were confirmed by biochemical tests. S. aureus isolates were further identified based on 23S rRNA, themonuclease and ENTEROTOXIN A GENEs using MULTIPLEX PCR. Based on MULTIPLEX PCR results all 55 isolates were identified as S. aureus. The ENTEROTOXIN A GENE (sea) was detected in 6 (10.9%) of the isolates. In conclusion, S. aureus and sea GENE was found in traditional white– brined cheese. It seems that if the favorable growth conditions are provided, S. aureus could proliferate and produce ENTEROTOXIN and could be regarded as a potential risk for human health.

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APA: Copy

MOHAMMADI, KH.. (2015). DISTRIBUTION OF STAPHYLOCOCCAL ENTEROTOXIN A GENE AMONG STAPHYLOCOCCUS AUREUS ISOLATES FROM TRADITIONAL WHITE-BRINED CHEESE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 11 (NEW)(4 (47)), 1473-1479. SID. https://sid.ir/paper/197359/en

Vancouver: Copy

MOHAMMADI KH.. DISTRIBUTION OF STAPHYLOCOCCAL ENTEROTOXIN A GENE AMONG STAPHYLOCOCCUS AUREUS ISOLATES FROM TRADITIONAL WHITE-BRINED CHEESE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2015;11 (NEW)(4 (47)):1473-1479. Available from: https://sid.ir/paper/197359/en

IEEE: Copy

KH. MOHAMMADI, “DISTRIBUTION OF STAPHYLOCOCCAL ENTEROTOXIN A GENE AMONG STAPHYLOCOCCUS AUREUS ISOLATES FROM TRADITIONAL WHITE-BRINED CHEESE,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 11 (NEW), no. 4 (47), pp. 1473–1479, 2015, [Online]. Available: https://sid.ir/paper/197359/en

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